Tumor Propagation
Tumor Propagation - Related Videos
05:22
Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model
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Cited by 8 •
2015
Circulating tumor cells (CTCs) have been shown to play an important role in tumor metastasis. Here, a method for the isolation and propagation of CTCs from the whole blood of a syngeneic mouse tumor model of hepatocellular carcinoma (HCC) metastasis is described.
07:55
Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
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Cited by 8 •
2011
A method is described to propagate human retinoblastoma tumors in mice. Tumor cells are directly injected into the eyes of immune deficient mice. Secondary tumors have been successfully established using both cells directly harvested from human tumors and cultured tumorspheres.
08:01
Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish
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Cited by 35 •
2011
Limiting dilution cell transplantation assays are used to determine the frequency of tumor-propagating cells. This protocol describes a method for generating syngeneic zebrafish that develop fluorescently-labeled leukemia and details how to isolate and transplant these leukemia cells at limiting dilution into the peritoneal cavity of adult zebrafish.
03:20
Splitting Spheroids for Sub-culturing and Shipping: A Procedure for Propagating and Transferring Spheroids from Small Bowel Neuroendocrine Tumor
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2023
This video demonstrates a strategy to split and propagate spheroids developed from small bowel neuroendocrine tumor cells. These spheroids have small bowel neuroendocrine tumor markers and can be propagated and further used to test anti-cancer drugs.
01:07
Propagation of Waves
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2023
When a wave propagates from one medium to another, part of it may get reflected in the first medium, and part of it may get transmitted to the second medium. In such a case, the interface of the two mediums can be considered as a boundary that is neither fixed nor free. Consider a scenario where a wave propagates from a string of low linear mass density to a string of high linear mass density. In such a case, the reflected wave is out of phase with respect to the incident wave, however the...
FAQs
Genetic and epigenetic changes can help abnormal cells maintain repeated proliferation and survive conditions that would otherwise limit their growth. These alterations may also support invasion, dissemination, and persistence during disease progression. Studying them gives researchers a way to connect cellular changes with aggressive behavior and may help identify biomarkers associated with progression, recurrence, or treatment resistance.
Invasion through the surrounding extracellular matrix allows abnormal cells to move beyond the tissue region where they initially developed. This step connects local growth with access to blood or lymphatic vessels and therefore with potential dissemination through the body. Examining matrix invasion helps researchers evaluate how effectively a tumor can extend into nearby tissue and progress toward metastatic disease.
Local propagation primarily reflects continued proliferation and survival within an existing tissue environment, whereas metastatic spread requires additional stages. Cells must invade surrounding matrix, enter blood or lymphatic vessels, leave circulation, and colonize a distant organ. Distinguishing these stages helps clarify why widespread disease represents a more complex pattern of propagation than expansion at the original site.
Propagation depends on whether abnormal cells can keep proliferating and survive as their surroundings change. Cells that tolerate changing conditions may persist while they invade tissue or move through circulation. Comparing behavior under different conditions can reveal which tumor characteristics support persistence and can help explain why some tumors progress, recur after treatment, or resist therapy.
Researchers study propagation by examining the cellular behaviors that sustain tumor growth and spread, including proliferation, survival, matrix invasion, vascular entry, and distant colonization. These investigations can use disease models to represent different stages of progression. The resulting observations support evaluation of biomarkers and therapies while improving understanding of how tumors change over time.
Studies of propagation can show how tumors continue growing, invade tissues, disseminate, or persist despite changing conditions. This information helps researchers evaluate therapies and investigate why treatment may fail to eliminate all disease. It also supports the search for biomarkers that indicate progression or resistance, making propagation research relevant to recurrence and the development of more informative treatment strategies.