Vaginal Balloon Dilation

Vaginal balloon dilation is a minimally invasive medical technique that gradually enlarges or creates a vaginal canal using an inflatable balloon, offering an alternative to more extensive reconstructive surgery in selected patients. During the procedure, clinicians position a deflated balloon within a surgically created or narrowed space and progressively inflate it under controlled pressure, allowing surrounding tissue to stretch and remodel over time. The method may support vaginal reconstruction for congenital vaginal agenesis and help manage postoperative or treatment-related stenosis. Careful sizing, pressure control, tissue monitoring, and follow-up are essential for comfort, healing, and maintaining the desired canal dimensions.

Vaginal Balloon Dilation - Related Videos

Research

JoVE Journal - Immunology and Infection

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

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Cited by 83 •

2011

Key techniques to be used in the evaluation of Candida vaginitis in an experimental animal model are described. The methods will allow rapid collection of vaginal specimens and lymphocytes from draining lumbar lymph nodes. These techniques could give rise to mouse models of other diseases in the female lower genital tract.

Modeling Murine Vaginal Colonization by Anaerobic Bacteria

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2025

Source: Morrill, S. R., et al. Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria. J. Vis. Exp. (2022)This video demonstrates a protocol for modeling vaginal colonization in mice using antibiotic-resistant anaerobic bacteria. It begins with hormonal treatment to increase epithelial susceptibility, followed by vaginal inoculation and co-inoculation under anaerobic conditions. The procedure concludes with post-inoculation sampling and culture to evaluate bacterial colonization...

A Murine Model of Group B Streptococcus Vaginal Colonization

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Cited by 64 •

2016

The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.

Establishing a Mouse Model of Vaginal Group B Streptococcus Infection

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2026

Source: Patras, K. A., Doran, K. S. A Murine Model of Group B Streptococcus Vaginal Colonization. J. Vis. Exp. (2016)This video demonstrates the preparation and vaginal inoculation of a hormone-treated female mouse with Group B Streptococcus to model infection. It explains how to culture and prepare the bacteria, administer the inoculum into the vaginal lumen, and ensure successful infection.

Research

JoVE Journal - Biology
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Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification

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Cited by 673 •

2012

Here, we describe how to identify the stage of the murine reproductive (proestrus, estrus, metestrus, or diestrus) by simple, non-invasive collection and cytological assessment of vaginal smear samples. We further describe how vaginal cytology reflects circulating hormonal levels underlying transition through the murine reproductive cycle.

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