Whole Lung Modeling
Whole Lung Modeling - Related Videos
05:49
Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation
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Cited by 5 •
2023
Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.
08:59
Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
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Cited by 27 •
2015
Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.
09:22
Mouse Pneumonectomy Model of Compensatory Lung Growth
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Cited by 14 •
2014
Mouse pneumonectomy is a commonly employed model of compensatory lung growth. This procedure can be used in conjunction with lineage tracing or transgenic mouse models to elucidate underlying mechanisms.
08:58
Development of a Neonatal Piglet Acute Lung Injury Model Recreating the Early Environment of Preterm Infant Lungs
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2025
This protocol describes the development of a neonatal piglet model of acute lung injury that models early pathogenic events occurring in the preterm lung, including sub-adequate surfactant quantity, hyperoxia, high-pressure ventilation, and inflammation, to facilitate understanding of molecular triggers of bronchopulmonary dysplasia and enhance therapeutic translation.
04:35
Ex vivo Pig Lung Model of Biofilm: A Technique to Develop Lung Model to Study Bacterial Biofilms on Pig Bronchiolar Tissue
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2025
This video describes the protocol for developing an ex vivo pig lung model of bacterial biofilm on infected bronchiolar tissue. Biofilms are bacterial aggregates embedded in a matrix adherent to a surface. This model is used to study the antibiotic susceptibility of bacteria at different stages of biofilm formation.
FAQs
The model links airway geometry, tissue mechanics, blood perfusion, and oxygen or carbon dioxide transport within one organ-wide framework. This connection allows a localized change, such as altered airway structure or tissue behavior, to be examined in relation to breathing, pressure, gas exchange, and broader lung performance rather than as an isolated regional event.
Airway geometry helps determine how airflow moves through the lung, while tissue mechanics represents how lung structures respond during breathing. Together, they help the model relate anatomical organization to airflow and pressure. Including both features makes it possible to study how structural or mechanical changes may influence function across the entire organ.
Blood perfusion and oxygen or carbon dioxide transport extend the analysis beyond airflow alone. Perfusion represents the blood-flow component of lung function, while gas transport addresses exchange involving respiratory gases. Combining these processes helps connect ventilation-related behavior with organ-wide gas exchange, providing a more complete physiological picture under defined model conditions.
Imaging data can provide information about lung anatomy, including airway geometry, while mathematical equations represent processes such as airflow, pressure, tissue mechanics, perfusion, and gas transport. These elements are integrated into a computational or physical representation and evaluated under defined conditions, allowing researchers to examine predicted respiratory behavior systematically.
A typical workflow begins by assembling a representation of lung anatomy, often using imaging data, and then incorporating equations for airflow, pressure, tissue mechanics, perfusion, and gas transport. Researchers define the conditions to be studied, run the computational or physical model, and examine how the simulated lung responds. The resulting patterns support organ-level interpretation.
Medical researchers can apply these models to investigate asthma, chronic obstructive pulmonary disease, acute respiratory distress, and other disorders. The approach helps relate disease-associated changes to whole-lung function and may inform ventilator settings or treatment planning. It also supports development of respiratory research tools designed to represent disease responses more predictively.