Cell Suspension Culture
Cell Suspension Culture - Related Videos
03:19
Generating an Organoid Culture from a Brain Tumor Cell Suspension
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2025
This video describes the preparation and cultivation of brain tumor organoids from single-cell suspension. Using a laminin-rich extracellular matrix as a scaffold and culture media for nutrients, cells are facilitated to multiply and self-organize within the organoid. Consequently, ex vivo organoids are generated, mimicking cellular diversity and the microenvironment of brain tumors.
11:44
Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
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Cited by 5 •
2013
Here we describe a robust method for the fractionation of plant plasma membranes into detergent resistant and detergent soluble membranes based on a mixture of unlabeled and in vivo fully 15N labeled Arabidopsis thaliana cell cultures. The procedure is applied for comparative proteomic studies to understand signaling processes.
13:10
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
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Cited by 3 •
2017
Here, it is demonstrated how the in situ Proximity Ligation Assay (PLA) can be used to detect and visualize the direct protein-protein interactions between ATM and p53 in suspension cell cultures exposed to genotoxic stress.
13:55
Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
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Cited by 40 •
2013
Vacuolar and cytosolic pH can be measured in live yeast (S. cerevisiae) cells using ratiometric fluorescent dyes localized to specific cellular compartments. We describe procedures for measuring vacuolar pH with BCECF-AM, which localizes to the vacuole in yeast, and cytosolic pH with a cytosolic ratiometric pH-sensitive GFP (yeast pHluorin).
11:17
Generation of Single-Cell Suspensions from Mouse Neural Tissue
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Cited by 16 •
2009
Dissociating cells from specific tissue types requires specific parameters for tissue aggitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS Dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on nerual tissue is explained.
FAQs
Gentle agitation keeps cells dispersed enough for the culture to remain suspended while the liquid medium supplies nutrients and supports gas exchange. This combination helps researchers maintain a controllable environment for expanding and analyzing neural cell populations, rather than allowing settling to dominate the experimental system.
Free-floating neurospheres provide a way to examine neural stem and progenitor cells as aggregates rather than only as dispersed cells. Under defined conditions, their formation can be incorporated into studies of neural development, proliferation, and differentiation. Researchers can therefore analyze how experimental conditions affect both population behavior and the emergence of neural cell states.
The main controllable features described are the liquid nutrient medium, gentle agitation, and defined conditions that may support neurosphere formation. Together, these features influence nutrient availability, gas exchange, suspension, and cellular organization. Adjusting them helps align the culture with expansion, developmental, or treatment-response analyses without changing the overall liquid-culture format.
A basic workflow begins by placing neural cells in a liquid nutrient medium, then applying gentle agitation to keep them suspended. Researchers can maintain the culture under defined conditions when studying neurosphere formation, while using the system to follow expansion or cellular responses. Subsequent analysis can focus on proliferation, differentiation, development, or treatment effects.
It is useful when researchers need to investigate neural development, cell proliferation, or differentiation in a controllable population-based system. The same approach can be used to examine how neural cells respond to experimental treatments. Its liquid format also supports studies requiring expansion of neural stem or progenitor cells before analysis.
Beyond developmental experiments, the method can support scalable production of neural cells and models for investigating disease mechanisms. It also contributes to research on potential regenerative strategies by providing a culture system in which neural stem or progenitor populations can be expanded, analyzed, and studied under defined experimental conditions.