Cx30 Promoter Recombination
Cx30 Promoter Recombination - Related Videos
14:23
Recombineering Homologous Recombination Constructs in Drosophila
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Cited by 9 •
2013
Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.
02:40
The Eukaryotic Promoter Region
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2020
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
03:38
A Method to Produce Recombinant Antibodies Against Metallopeptidase
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2025
This video demonstrates a procedure for the production of recombinant antibodies against metallopeptidase. Recombinant plasmids carrying genes for the antibody, green fluorescent protein, and antibiotic resistance are transfected into epithelial cells with lipofectamine. The transfected cells are selected and cultivated to generate recombinant antibodies.
10:58
Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
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Cited by 15 •
2017
Promoter expression analyses are crucial to improving the understanding of gene regulation and the spatiotemporal expression of target genes. Herein we present a protocol to identify, isolate, and clone a plant promoter. Further, we describe the characterization of the nodule-specific promoter in the common bean hairy roots.
08:56
Recombineering and Gene Targeting
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2023
One of the most widely used tools in modern biology is molecular cloning with restriction enzymes, which create compatible ends between DNA fragments that allow them to be joined together. However, this technique has certain restrictions that limit its applicability for large or complex DNA construct generation. A newer technique that addresses some of these shortcomings is recombineering, which modifies DNA using homologous recombination (HR), the exchange between different DNA molecules based...
FAQs
Recombination is linked to activity of regulatory DNA from Cx30, also called Gjb6. Cells in which that promoter is active can produce Cre, whereas cells outside that expression pattern are not intended to activate the same conditional allele. In neuroscience, this links genetic manipulation to Cx30-expressing astrocytes and helps investigators relate molecular changes to astrocyte-associated functions.
The allele's loxP arrangement determines the genetic consequence after Cre recognition. Depending on how those sites are positioned around the conditional sequence, recombination can remove that sequence or enable a previously conditional reporter or manipulation. Thus, the same promoter-driven recombinase strategy can support either genetic deletion or expression, rather than producing one fixed outcome.
The strategy provides a cell-directed comparison: a change produced through Cx30-linked recombination can be evaluated against effects from a broader genetic manipulation. If the outcomes differ, the contrast helps identify contributions associated with Cx30-expressing astrocytes rather than attributing the result to all affected cell populations. This distinction is especially relevant for interpreting circuit and synaptic phenotypes.
A knockout tests the consequence of removing or altering a conditional gene, while a reporter marks the cells reached by the recombination strategy. Lineage tracing extends that information by documenting the relevant cellular population during experimental analysis. Used together, these readouts help connect genetic targeting with astrocyte identity and with the resulting neural phenotype.
A typical design places Cre recombinase under Cx30 regulatory control and pairs that construct with a conditional allele containing loxP sites. The selected allele then determines whether the experiment produces deletion, activation, or labeling. Researchers can therefore tailor the same targeting logic to a reporter, a knockout, or another conditional manipulation in Cx30-expressing astrocytes.
By directing a reporter or genetic manipulation to Cx30-expressing astrocytes, investigators can examine how these cells relate to synaptic regulation and neural circuits. The approach also supports studies of brain disease, where cell-type-directed changes may clarify astrocyte contributions. Its value lies in connecting a defined astrocyte population with observable molecular or circuit-level outcomes.