Dugesia Dorotocephala

Dugesia dorotocephala is a freshwater planarian, or flatworm, whose compact centralized nervous system and remarkable regenerative capacity make it a useful model for neuroscience. Its cephalic ganglia connect to paired longitudinal nerve cords and peripheral branches, coordinating sensory input, locomotion, and responses to environmental cues; after injury, neural tissues can be rebuilt through proliferative stem cells and tissue remodeling. Researchers use this species to study neural regeneration, brain organization, learning and memory, and the relationship between nervous-system structure and behavior. These experiments provide an accessible framework for examining fundamental principles of repair and nervous-system function.

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JoVE Journal - Biology
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Pharmacological and Functional Genetic Assays to Manipulate Regeneration of the Planarian Dugesia japonica

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2011

An attractive model for studying stem cell differentiation within a live animal is the planarian flatworm. Regeneration is studied by simple amputation experiments that are easily performed in a basic laboratory and are amenable to pharmacological and genetic (in vivo RNAi) manipulation as detailed by protocols in this article.

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