Hippocampal Slice Cultures
Hippocampal Slice Cultures - Related Videos
08:20
Organotypic Hippocampal Slice Cultures
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Cited by 52 •
2011
We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.
06:33
The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury
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Cited by 9 •
2010
The organoptypic hippocampal slice culture model is an in vitro model used to examine neuronal injury in a variety of paradigms. In this article, we describe the methods for generating slice cultures and quantifying neuronal injury.
05:33
Gene Expression via Single-Cell Electroporation in Mouse Hippocampal Slice Cultures
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2025
Source: Keener, D. G., et al. Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons. J. Vis. Exp. (2020).This video demonstrates the process of single-cell electroporation to introduce plasmid DNA into neurons within mouse hippocampal slice cultures. The procedure involves precise pipette positioning, pressure adjustments, and electroporation pulses to transiently permeabilize the neuronal membrane,...
07:48
Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
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Cited by 18 •
2017
Organotypic hippocampal slice cultures (OHSC) represent an in vitro model that simulates the in vivo situation very well. Here we describe a vibratome-based improved slicing protocol to obtain high quality slices for use in assessing the neuroprotective potential of novel substances or the biological behavior of tumor cells.
13:50
Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
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Cited by 13 •
2013
The localization and distribution of proteins provide important information for understanding their cellular functions. The superior spatial resolution of electron microscopy (EM) can be used to determine the subcellular localization of a given antigen following immunohistochemistry. For tissues of the central nervous system (CNS), preserving structural integrity while maintaining antigenicity has been especially difficult in EM studies. Here, we adopt a procedure that has been used to preserve...
FAQs
Positioning a tissue slice on a porous membrane creates an air–liquid interface that helps nutrients and gases reach the preparation while it remains under laboratory conditions. This arrangement supports the survival and activity of neurons and glial cells, allowing researchers to examine local synaptic communication without working with an intact organism.
Retaining much of the hippocampus’s native cellular organization and local neural circuitry preserves interactions among nearby neurons and glial cells. Those relationships allow experiments to examine circuit activity and synaptic communication in a controlled setting. As a result, researchers can connect cellular or synaptic changes with mechanisms relevant to hippocampal function and learning-related processes.
The controlled ex vivo environment permits researchers to introduce drugs, apply genetic manipulations, or use electrophysiological methods while observing hippocampal tissue. These interventions can alter or measure neural activity under defined laboratory conditions. Comparing treated and untreated preparations helps investigators examine mechanisms of synaptic plasticity, responses to injury or disease, and potential therapeutic effects.
Because the tissue is maintained outside the organism, researchers can control experimental exposure and directly study hippocampal cells and local circuitry. This focus makes it easier to relate an intervention to synaptic communication, circuit activity, or cellular responses without the full complexity of an intact animal. The model therefore complements, rather than replaces, broader neuroscience approaches.
Researchers prepare thin sections of hippocampal tissue and maintain them under laboratory conditions on a porous membrane. The membrane supports an air–liquid interface, enabling access to nutrients and gases while the tissue remains positioned for observation and experimentation. Once maintained, the slices can receive drugs, genetic interventions, or electrophysiological analysis.
This preparation is useful when investigators need controlled access to hippocampal tissue while studying neuronal development, synaptic plasticity, learning-related mechanisms, or responses to injury and disease. It also supports testing of potential therapeutic strategies. Its combination of preserved local organization and experimental control makes it relevant to both basic circuit studies and intervention-focused research.