Homotopic Grafting
Homotopic Grafting - Related Videos
01:03
¹H NMR Chemical Shift Equivalence: Homotopic and Heterotopic Protons
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2024
Protons in identical electronic environments within a molecule are chemically equivalent and have the same chemical shift. The replacement test is a useful tool to identify chemical equivalence and predict NMR spectra. A substituent replaces each of the protons being examined and the resulting molecules are compared. If the same molecule is obtained, the protons are equivalent or homotopic. Replacement of any hydrogens in ethane by chlorine yields chloroethane because all six protons are...
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
03:35
A Technique for Human Skin Grafting in a Mouse Model
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2025
This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse's graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse's skin, establishing vascularization and circulation, resulting in a successful skin xenograft.
05:47
Isolation and Transplantation of Different Aged Murine Thymic Grafts.
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Cited by 14 •
2015
This report provides a detailed description of transplanting murine thymi from different aged donor mice under the kidney capsule of immunodeficient mouse recipients. The goal of this approach is to model T cell development and thymic selection events in vivo.
13:04
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
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Cited by 13 •
2015
Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.
FAQs
Local signals in the recipient region can influence transplanted cells after placement, shaping their survival, differentiation, and migration. These effects represent extrinsic influences, meaning cues supplied by surrounding tissue, whereas intrinsic properties arise from the donor cells themselves. Comparing matched placements helps researchers assess how strongly the environment contributes to neural cell behavior.
Preserving the corresponding site gives transplanted tissue access to region-specific surroundings rather than exposing it to an unrelated neural environment. This matters because local context may alter developmental behavior and circuit relationships. A homotopic placement therefore supports cleaner interpretation of whether observed outcomes reflect donor characteristics, recipient cues, or their interaction.
Researchers can examine several outcomes, including transplanted-cell survival, differentiation, migration, connectivity, and relationships with existing neural circuits. Considering these processes together helps distinguish whether grafted tissue remains viable, develops relevant characteristics, reaches appropriate locations, and participates in circuit organization. The approach therefore connects cellular behavior with broader questions about neural development and repair.
Suitable donor material may include embryonic neural tissue, stem-cell-derived cells, or other neural tissue, depending on the experimental question. The workflow requires identifying the recipient region corresponding to the donor’s intended site, placing the material there, and evaluating responses such as survival, differentiation, migration, or circuit-related changes. These readouts connect transplantation conditions with biological outcomes.
After injury or disease, researchers can use this approach to evaluate cell replacement strategies in a spatially appropriate neural setting. They may examine whether transplanted tissue survives, develops, connects with existing circuitry, or contributes to restored function. Matching the recipient location helps relate these outcomes to the damaged region and provides context for judging repair potential.
Interpretation should consider both the behavior of the donor tissue and the influence of its recipient environment. Evidence about survival, differentiation, migration, connectivity, or function can indicate different stages of graft response, but no single outcome fully describes integration. Evaluating several measures together helps clarify how transplanted cells interact with local neural circuitry and developmental signals.