Horizontal Brain Slice
Horizontal Brain Slice - Related Videos
02:55
Obtaining Horizontal Mouse Hippocampal Brain Slices
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2025
This video demonstrates a detailed protocol for preparing horizontal hippocampal brain slices from mice, focusing on preserving the integrity of hippocampal fiber pathways. The process involves carefully bisecting and securing the brain, using a vibratome for slicing, and maintaining optimal tissue conditions with a carbogenated fluid, resulting in hippocampal brain slices ready for further experimentation.
08:59
Horizontal Hippocampal Slices of the Mouse Brain
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Cited by 12 •
2020
This article aims to describe a systematic protocol to obtain horizontal hippocampal brain slices in mice. The objective of this methodology is to preserve the integrity of hippocampal fiber pathways, such as the perforant path and the mossy fiber tract to assess dentate gyrus related neurological processes.
10:45
Horizontal Slice Preparation of the Retina
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Cited by 4 •
2006
Traditionally the vertical slice and the whole-mount preparation of the retina have been used to study the function of retinal circuits. Here, we describe the novel slicing method to preserve the dendritic morphology of retinal neurons intact.
07:02
Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies
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Cited by 14 •
2017
We developed a horizontal slice preparation of the adult mammalian retina with the plane of sectioning parallel to the retinal surface. Dendritic fields of nonradially oriented retinal neurons were not truncated, allowing the study of signal processing with patch-clamp and imaging techniques.
06:33
Preparing Wedge Brain Slices to Visualize Auditory Neurons
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2025
This video demonstrates the process of preparing wedge-shaped brain slices in a laboratory setting. Using a vibratome, a wedge-shaped slice from a mouse's brain is crafted with varying thickness. This slice contains the auditory nerve root positioned on the thicker end, while the hearing-related neurons are located on the thinner end.
FAQs
Cutting tissue parallel to the brain's base retains relationships among interconnected neural structures in a horizontal plane while producing a flat preparation. This geometry helps investigators examine activity across anatomically defined pathways rather than limiting measurements to isolated cellular elements. The result supports circuit-level analysis alongside cellular electrophysiological or imaging measurements.
Rapid sectioning in chilled, oxygenated artificial cerebrospinal fluid helps preserve physiological responsiveness after tissue removal. Chilling is part of the preparation conditions, while oxygenation supports maintenance of the slice in an artificial environment. Keeping these conditions controlled allows neurons and synapses to remain suitable for studying transmission and circuit activity.
Because the cut surface exposes neural tissue, investigators can place electrophysiological access and imaging measurements close to neurons and synapses. This accessibility makes it possible to assess synaptic transmission, circuit activity, and responses to pharmacological manipulation in the same general preparation. Measurements can therefore connect cellular behavior with activity in anatomically defined pathways.
A typical preparation begins with tissue removal, followed by rapid sectioning in chilled, oxygenated artificial cerebrospinal fluid. The resulting slice is then maintained under controlled conditions before measurements are performed. This sequence is important because the preparation must retain responsive neurons and synapses while giving investigators access to the exposed tissue for electrophysiology or imaging.
Researchers select this preparation when they need to examine connectivity, synaptic transmission, or pharmacological responses in preserved tissue architecture. The slice provides a controlled setting in which cellular mechanisms can be studied alongside activity across defined pathways. It is therefore useful for linking local neuronal or synaptic effects to broader circuit behavior rather than examining isolated cells alone.
By preserving anatomically defined pathways while keeping neurons and synapses physiologically responsive, these experiments can reveal disease-related changes at both cellular and circuit levels. Investigators may compare synaptic transmission, connectivity, or activity under controlled conditions and examine how pharmacological responses differ. Such observations help relate altered cellular mechanisms to functional changes in neural circuits.