Journal
/
Biology
/
Horizontal Slice Preparation of the Retina
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Horizontal Slice Preparation of the Retina

Horizontal Slice Preparation of the Retina

DOI:
10.3791/108-v

•

10:45 min

•

November 20, 2006

•

, ,
1Dpt of Physiology and Biophysics,Dalhousie University, 2Massachusetts General Hospital,Harvard Medical School

Chapters

  • 00:05Tools
  • 00:22Preparation of agar block and agarose gel
  • 00:51Isolation of retina
  • 02:06Preparation of retina slices
  • 10:17Examples: cell layer of retina

Summary

Automatic Translation

Automatic Translation

Traditionally the vertical slice and the whole-mount preparation of the retina have been used to study the function of retinal circuits. Here, we describe the novel slicing method to preserve the dendritic morphology of retinal neurons intact.

Tags

Horizontal SliceRetinaRetinal CircuitsAmacrine CellsDendritesVertical SliceWhole-mount PreparationMorphologyPatch-clamp RecordingsGlial CellsMueller CellsSlicing MethodVibratome SlicerLow-temperature Melting Agarose GelFunction Of Retinal NeuronsCalcium Imaging TechniqueImmunocytochemistrySingle-cell RT-PCR

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/108?article_type=v

Light/dark Transition Test for Mice
check_url/108?article_type=v

Organotypic Culture of Adult Rabbit Retina
check_url/108?article_type=v

Thin Sectioning of Slice Preparations for Immunohistochemistry
check_url/108?article_type=v

Organotypic Slice Culture of E18 Rat Brains
check_url/108?article_type=v

Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
check_url/108?article_type=v

Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice
check_url/108?article_type=v

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses
check_url/108?article_type=v

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
check_url/108?article_type=v

In Ovo Electroporation in Embryonic Chick Retina
check_url/108?article_type=v

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

Read Article