Murine Lung Histology
Murine Lung Histology - Related Videos
10:01
Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation
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Cited by 20 •
2012
Obliterative bronchiolitis is the key impediment to the long-term survival of lung transplant recipients and the lack of a robust preclinical model precludes examining obliterative bronchiolitis immunopathogenesis. Unlike other solid organ transplants, vascularized mouse lung transplantation has only recently been developed. Here we show our independently developed obliterative bronchiolitis model after murine orthotopic single-lung transplantation.
04:24
Perfusion and Inflation of the Mouse Lung for Tumor Histology
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Cited by 34 •
2020
The purpose of this method is to present a simple and efficient method for the perfusion, inflation, and fixation of mouse lungs for the examination of lung tumor pathology and evaluation of metastases to the lung.
02:37
Cryopreservation of Murine Brain by Snap Freezing: A Technique to Rapidly Freeze Whole Mouse Brain Specimen for Histological Analysis
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2023
This video demonstrates the cryopreservation of a mouse’s brain to isolate discrete anatomical areas or specific cell populations from the tumor tissues. This technique is critical in obtaining optimal optical resolution of brain tissue morphology for subsequent analysis.
05:54
An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs
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2025
This video demonstrates an in vitro technique to identify immune cells residing in murine lungs. After isolating target immune cells from murine lung tissue, they are surface-labeled using a cocktail of fluorophore-tagged antibodies against specific markers on innate and adaptive immune cells. Additionally, monocytes, macrophages, and dendritic cells are labeled using an intracellular marker. The various immune cell types are then identified using flow cytometry.
09:52
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
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Cited by 11 •
2018
Here we describe a protocol to determine pulmonary fungal burden in mice with invasive aspergillosis by quantification of Gomori's modified methanamine silver staining in histological sections. Use of this method resulted in comparable results with less animals compared to assessment of fungal burden by quantitative PCR of lung fungal DNA.
FAQs
These stages prepare lung samples so microscopic examination can reveal preserved architecture and pathological changes. Light microscopy then makes features such as airways, alveoli, blood vessels, inflammation, and fibrosis visible. Applying the stages consistently allows researchers to examine comparable tissue sections across experimental conditions and relate microscopic findings to the study’s broader biological question.
Airways, alveoli, and blood vessels provide complementary morphological landmarks. Examining them together helps researchers determine where an experimental effect is located within the lung rather than relying on a single tissue feature. The same sections may also reveal inflammatory or fibrotic alterations, broadening interpretation from normal architecture to disease-associated change.
Morphological evidence shows what tissue looks like, whereas functional measurements address how the system performs. Comparing both types of data can reveal whether an apparent physiological change is accompanied by structural alteration. This combined interpretation is especially useful when studying pulmonary effects of neural injury, neuroimmune signaling, or experimental therapies.
Researchers proceed from fixation through tissue processing, sectioning, staining, and light-microscopic examination, then compare the resulting sections across experimental groups. This sequence creates a consistent path from tissue collection to morphological assessment. Group comparisons can identify differences in lung architecture or pathological change and provide evidence that complements other experimental measurements.
They are particularly relevant when a study examines pulmonary consequences of neural injury, neuroimmune signaling, or an experimental therapy. In these settings, stained sections can show whether respiratory tissue exhibits architectural or pathological changes. The resulting tissue-level observations add context to investigations in which the lung is an important component of a broader neuroscience study.
It enables researchers to examine respiratory tissue alongside a neuroscience-focused intervention or condition. By comparing lung morphology among experimental groups, investigators can assess inflammatory, fibrotic, or architectural changes in relation to the broader study question. This approach helps connect neural or neuroimmune phenomena with systemic disease mechanisms when pulmonary tissues are involved.