Nanoliter 2000 Injector
Nanoliter 2000 Injector - Related Videos
05:06
Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector
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Cited by 10 •
2021
This protocol provides detailed guidance for the initial and continued generational allotransplantation of Drosophila tumors into the abdomen of adult hosts for studying various aspects of neoplasia. Using an autoinjector apparatus, researchers can achieve improved efficiency and tumor yields compared to those achieved by traditional, manual methods.
07:46
Assessing Endothelial Vasodilator Function with the Endo-PAT 2000
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Cited by 165 •
2010
A noninvasive procedure to assess endothelial function is demonstrated using the Endo-PAT 2000.
13:36
Scalable Fluidic Injector Arrays for Viral Targeting of Intact 3-D Brain Circuits
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Cited by 10 •
2010
Controlling and analyzing neural circuits in vivo would be facilitated by a technology for delivery of viruses and other reagents to desired 3-dimensional sets of brain regions. We demonstrate customized fluidic injector array fabrication, and delivery of virally-encoded optical sensitizers, enabling optical manipulation of complex brain circuits.
09:32
LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
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Cited by 66 •
2013
Ice binding proteins (IBPs), also known as antifreeze proteins, inhibit ice growth and are a promising additive for use in the cryopreservation of tissues. The main tool used to investigate IBPs is the nanoliter osmometer. We developed a home-designed cooling stage mounted on an optical microscope and controlled using a custom-built LabVIEW routine. The nanoliter osmometer described here manipulated the sample temperature in an ultra-sensitive manner.
09:39
Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
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Cited by 6 •
2012
A rapid in vivo assay to test for neuromodulatory compounds using the Giant Fiber System (GFS) of Drosophila melanogaster is described. Nanoinjections in the head of the animal along with electrophysiological recordings of the GFS can reveal bioactivity of compounds on neurons or muscles.
FAQs
The instrument combines regulated pressure with timed injection pulses to determine how much solution passes through the micropipette during each delivery. Pressure provides the driving force, while pulse timing helps constrain the delivered volume. This coordination supports repeatable dosing when an experiment requires localized administration and minimal disturbance to nearby cells or tissue.
The fine glass micropipette provides the narrow delivery path needed to direct a measured solution into a selected cell, brain region, or experimental preparation. Its use supports spatially controlled administration rather than broad fluid application. In neuroscience experiments, this focused delivery helps connect a treatment or label with a particular neural location and limits unintended spread.
Small, controlled doses can reduce physical and chemical disturbance around the target while preserving the ability to administer a defined solution. This is valuable when researchers need to distinguish local effects from effects caused by widespread exposure. Consistent delivery also improves reproducibility across experiments that examine cellular responses, neural circuits, or targeted interventions.
A basic workflow centers on placing the solution in a fine glass micropipette, positioning the pipette at the intended cellular or tissue target, and applying regulated pressure for a selected pulse duration. The measured delivery is then directed into the preparation. This workflow links physical placement and controlled timing to localized dosing, labeling, or intervention.
The approach can deliver dyes, tracers, pharmacological agents, genetic materials, and other solutions, provided they are compatible with the experimental preparation and micropipette delivery. The choice depends on the biological question: dyes and tracers can support localization, whereas pharmacological or genetic materials can help probe neural function and cellular responses at selected sites.
In neuroscience, researchers can use localized injections to examine neural circuits, observe cellular responses, or assess the consequences of a targeted intervention. Delivery may occur into individual cells, brain regions, or other experimental preparations. By limiting the administered volume and directing it to a defined site, the method helps relate observed outcomes to a specific neural location.