Neuroepithelium Imaging

Neuroepithelium imaging is the visualization of neuroepithelial tissue, an organized layer of neural progenitor cells that gives rise to the developing nervous system. Using microscopy to capture cell shape, tissue architecture, proliferation, migration, and differentiation, researchers can relate dynamic cellular behavior to changes in neuroepithelial structure over time. In neuroscience, these approaches help clarify how neural tissues form and become patterned during development, while also supporting studies of developmental disorders, injury, and stem cell-derived neural organoids. Quantitative imaging of cell position, division, and tissue organization provides evidence for mechanisms that regulate nervous system formation and may improve models of human neurological disease.

Neuroepithelium Imaging - Related Videos

Research

JoVE Journal - Neuroscience
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High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium

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Cited by 21 •

2012

Imaging embryonic tissue in real-time is challenging over long periods of time. Here we present an assay for monitoring cellular and sub-cellular changes in chick spinal cord for long periods with high spatial and temporal resolution. This technique can be adapted for other regions of the nervous system and developing embryo.

Research

JoVE Journal - Neuroscience

Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium

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Cited by 3 •

2013

Here we develop the tools necessary for ex vivo live imaging to trace single cell divisions in the mouse E8.5...

An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium

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Cited by 11 •

2012

We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.

Live Imaging of the Drosophila Pupal Eye Using Fluorescence Microscopy

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2025

Source: Hellerman, M. B., et al. Live-imaging of the Drosophila Pupal Eye. J. Vis. Exp. (2015)The video demonstrates a live-imaging technique to study eye patterning in a Drosophila melanogaster pupa. Green fluorescent protein (GFP)-tagged markers enable visualization of cellular organization and differentiation during the formation of the developing eye structure.

Hybrid µCT-FMT imaging and image analysis

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Cited by 34 •

2015

We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.

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