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High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium
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Chapters
- 00:05Title
- 01:33Embryo Electroporation
- 03:04Collagen and Slice Culture Medium Preparation
- 04:22Slice Culture Preparation
- 07:52Imaging Embryo Slices
- 08:58Results: Time-lapse Sequences of Spinal Cord Progenitor Cells
- 10:32Conclusion
Imaging embryonic tissue in real-time is challenging over long periods of time. Here we present an assay for monitoring cellular and sub-cellular changes in chick spinal cord for long periods with high spatial and temporal resolution. This technique can be adapted for other regions of the nervous system and developing embryo.
Tags
High-resolutionLive ImagingCell BehaviorDeveloping NeuroepitheliumEmbryonic Spinal CordCycling Neural Progenitor CellsNeuronal DifferentiationMolecular MechanismsNeurogenesisReal-time ImagingCell ViabilityCulturePhototoxic EffectsFluorescent ImagingEx Vivo Slice Culture ProtocolWide-field Fluorescence MicroscopyTime-lapse MonitoringSpatial ResolutionTemporal ResolutionCellular BehaviorsSub-cellular BehaviorsGene Activity ReportersNotch Signaling
