Three-dimensional Bioreactor
Three-dimensional Bioreactor - Related Videos
12:39
Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
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Cited by 7 •
2008
We describe a chip-based platform for the three-dimensional cultivation of cells in micro-bioreactors. One chip can house up to 10 Mio. cells that can be cultivated under precisely defined conditions with regard to fluid flow, oxygen tension etc. in a sterile, closed circulation loop.
12:54
Operation of a Benchtop Bioreactor
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Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
11:11
Construction and Characterization of a Novel Vocal Fold Bioreactor
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Cited by 8 •
2014
A novel vocal fold bioreactor capable of delivering physiologically relevant, vibratory stimulation to cultured cells is constructed and characterized. This dynamic culture device, when combined with a fibrous poly(ε-caprolactone) scaffold, creates a vocal fold-mimetic environment that modulates the behaviors of mesenchymal stem cells.
08:04
Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering
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Cited by 24 •
2013
We designed a novel mechanical loading bioreactor that can apply uniaxial or biaxial mechanical strain to a cartilage biocomposite prior to transplantation into an articular cartilage defect.
FAQs
Nutrient delivery and oxygenation help cells survive and remain active throughout a three-dimensional culture rather than only near its exposed surfaces. Fluid flow and mixing distribute these resources through scaffolds, aggregates, or organoids. Regulating these conditions supports more uniform growth and helps maintain neural tissue models for subsequent developmental, injury, disease, or drug-related studies.
Fluid flow improves the movement of nutrients and oxygen through the culture, while mechanical stimulation provides physical input that cells would encounter in a tissue environment. Together, these factors can influence how cells grow, organize, and communicate. Their inclusion makes the model more physiologically relevant than a culture that receives only a static, two-dimensional environment.
Conventional two-dimensional cultures grow cells on a flat surface, whereas three-dimensional systems support cells within structures such as scaffolds, aggregates, or organoids. This added spatial organization allows cells to interact throughout a tissue-like environment. For neuroscience research, the resulting models can better represent aspects of brain-like tissue organization and support more relevant experimental observations.
Scaffolds, aggregates, and organoids provide different structural settings in which neural cells or engineered brain-like tissues can grow. They create three-dimensional spaces that support cellular organization and communication while the bioreactor regulates surrounding conditions. Choosing among these formats allows researchers to study neural cultures in models suited to tissue-engineering, developmental, injury, disease, or drug-testing questions.
Preparation focuses on establishing the three-dimensional culture format and controlling the surrounding conditions that affect cell behavior. Key regulated factors include nutrient delivery, oxygenation, fluid flow, mixing, and mechanical stimulation. Maintaining these features helps cells grow and organize throughout the selected scaffold, aggregate, or organoid, producing a model suitable for downstream neuroscience experiments.
Researchers can apply these systems to study neural development, injury, and disease using neural cells or engineered brain-like tissues. The three-dimensional setting supports investigation of how cells organize and communicate under regulated culture conditions. It also provides a platform for tissue-engineering studies, where researchers evaluate the growth and organization of neural tissue models.
Three-dimensional neural cultures can provide drug-testing models that reproduce more physiologically relevant features than conventional two-dimensional cultures. Cells grow and communicate within a structured environment while nutrient delivery, oxygenation, and fluid conditions are regulated. This can make experimental observations more representative of responses in brain-like tissue and support evaluation of candidate treatments in disease-related models.