Renal Tubule
Renal Tubule - Related Videos
07:06
Isolation of Primary Human Proximal Tubule Epithelial Cells and Their Use in Creating a Microphysiological Model of the Renal Proximal Tubule
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Cited by 3 •
2025
We present an optimized protocol for processing whole human kidneys to isolate and culture primary renal proximal tubule epithelial cells and the application of these cells in a three-dimensional, microfluidic, microphysiological platform to recapitulate the renal proximal tubule.
01:24
Renal Tubule and Collecting Duct
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2024
The renal tubule is divided into three parts: the proximal convoluted tubule (PCT), the Loop of Henle (LOH), and the distal convoluted tubule (DCT). Proximal Convoluted Tubule (PCT): The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
03:21
Murine Renal Cell Isolation: A Technique to Isolate and Generate Primary Culture of Renal Tubular Epithelial Cells
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2023
In this video, we demonstrate the steps to isolate and purify renal tubular epithelial cells for a wide range of molecular biology and cell culture studies.
06:38
Early Detection of Drug-Induced Renal Hemodynamic Dysfunction Using Sonographic Technology in Rats
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Cited by 14 •
2016
Early stage hemodynamic dysfunction is critical to the development of kidney disease. Yet, detection methodologies are limited. Recent advances in sonography provide a noninvasive, accurate option for early detection of kidney injury. This study outlines a step-by-step, sonographic methodology for detecting kidney dysfunction using a drug-induced nephrotoxicity rat model.
04:25
Isolation of Immune Regulatory Sertoli Cells from Mouse Seminiferous Tubules
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2025
This video demonstrates the isolation procedure of Sertoli cells from mouse seminiferous tubules, a component of the testes. Enzymatic digestion and repeated washes promote the release of Sertoli cells into the solution, which can be cultured further for proliferation and growth.
FAQs
Segment location determines which transport processes a drug can influence. The proximal tubule, loop of Henle, distal tubule, and collecting duct contain distinct combinations of transporters and ion channels, so effects are not uniform along the nephron. This segment-specific organization helps explain why pharmacologic interventions can change fluid, electrolyte, or drug handling in different ways.
Hormonal signals fine-tune transport after filtrate has entered the tubule. Aldosterone adjusts sodium balance, while antidiuretic hormone changes permeability within the tubular system, thereby influencing water handling. These regulatory effects are pharmacologically important because they can modify the renal response to treatments that alter excretion, fluid status, or electrolyte balance.
Transporters and ion channels provide the molecular routes through which epithelial cells move water and solutes. Changes in these components can therefore alter tubular reabsorption or secretion and modify the amount of a substance excreted. Their segment-specific distribution gives pharmacologic agents defined renal targets and helps connect molecular action with changes in urine formation.
These processes describe different fates for medicines within tubular cells. Secretion moves a drug into the tubular pathway, reabsorption returns material from the filtrate toward the body, and accumulation reflects retention within tubular cells. Distinguishing among them helps explain how the renal tubule controls drug excretion and why it matters in toxicity assessment.
Tubular handling affects how medicines are secreted, reabsorbed, or retained in tubular cells, which can change renal excretion. Pharmacologic assessment therefore considers segment-specific transport when evaluating how a treatment may behave in the kidney. This information supports drug dosing decisions and helps identify circumstances in which tubular handling could contribute to toxicity.
They are particularly relevant when medicines are intended to alter renal excretion or manage conditions involving fluid and sodium balance. Segment-specific actions help explain the effects of diuretics used in hypertension and edema, while tubular transport mechanisms also inform treatment of electrolyte disorders. The same framework supports evaluation of drug handling and renal toxicity.