Reversible Irreversible
Reversible Irreversible - Related Videos
01:14
Reversible and Irreversible Processes
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2023
The thermodynamic processes can be classified into reversible and irreversible processes. The processes that can be restored to their initial state are called reversible processes. It is only possible if the process is in quasi-static equilibrium, i.e., it takes place in infinitesimally small steps, and the system remains at equilibrium However, these are ideal processes and do not occur naturally. An ideal system undergoing a reversible process is always in thermodynamic equilibrium within...
14:05
A Murine Model of Irreversible and Reversible Unilateral Ureteric Obstruction
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Cited by 47 •
2014
The murine model of irreversible unilateral ureteric obstruction (UUO) is presented together with the model of reversible UUO in which the ureteric obstruction is relieved by anastomosis of the severed ureter into the bladder. These models enable the study of renal inflammation and scarring as well as tissue remodeling.
03:50
Irreversible Electroporation: A Technique for Ablating Pancreatic Tumor in Mouse
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2023
This video describes the procedure for performing irreversible electroporation (IRE) for non-thermal ablation of pancreatic tumors in mice. Both subcutaneous (SQ) and orthotopic tumors can be successfully treated with IRE in a highly controlled setting, facilitating various studies post-procedure.
10:10
Reverse Total Shoulder Arthroplasty
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Cited by 5 •
2011
Reverse total shoulder arthroplasty is indicated for the treatment of conditions that cannot be treated with conventional arthroplasty or other procedures. These primarily include degenerative and incapacitating conditions with irreparable rotator cuff and loss of the normal biomechanical coupling of the shoulder.
06:17
Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
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2025
The quantitative irreversible tethering (qIT) assay is a fluorescence-based method of identifying covalent fragments that selectively engage with a target protein. Here, a detailed protocol is outlined to describe the qIT assay, with example results included.
FAQs
The interaction type determines how readily a drug-target complex changes over time. Ionic, hydrogen-bonding, and hydrophobic forces can permit dissociation, so the effect may decline as the drug leaves the target or is eliminated. Covalent modification creates a more persistent association, making the effect less dependent on the drug’s continued presence in the surrounding system.
Recovery depends on what happens to the modified target after drug exposure. With reversible binding, function can return as the drug dissociates or is eliminated. After irreversible modification, recovery may require the target to be replaced or regenerated. This difference helps explain why the pharmacological effect can persist even after the initiating drug is no longer present.
Binding reversibility contributes to both the apparent strength and persistence of a drug effect. A reversible interaction can lose influence when target occupancy decreases, whereas an irreversible interaction may continue affecting the target after drug elimination. Consequently, potency, duration of action, and the time required for recovery must be interpreted together rather than treated as independent properties.
Dose-response behavior reflects not only how much drug reaches a target but also whether the interaction can be disrupted. Reversible binding allows changes in drug concentration and dissociation to influence the response more directly. Irreversible binding can produce a more persistent effect, so the observed response may continue after exposure changes and may not track current drug concentration alone.
Researchers can interpret the persistence of inhibition in relation to drug removal or elimination and subsequent recovery. Declining inhibition as the drug dissociates supports a reversible interaction, while continued inhibition suggests a more persistent target modification. Applying this distinction to receptors and enzymes helps clarify whether the measured pharmacological effect depends on ongoing drug-target association or lasting target alteration.
Drug designers consider binding persistence when evaluating desired effects, dosing strategies, and potential safety concerns. A reversible interaction may allow effects to decrease as exposure falls, whereas an irreversible interaction may require target replacement or regeneration before recovery. Recognizing these differences supports interpretation of duration, dose-response behavior, and the consequences of prolonged target inhibition.