Retina Photoreceptors

Retina photoreceptors are specialized light-sensitive cells that convert incoming photons into neural signals, forming the first stage of visual processing. Rods support vision in dim conditions, while cones respond to brighter light and provide color and fine-detail perception; when light activates their opsins, intracellular signaling reduces neurotransmitter release through phototransduction. These signals pass through bipolar and ganglion cells before traveling through the optic nerve to the brain, where they contribute to visual perception and behavior. Studying photoreceptors helps psychology and neuroscience explain processes such as color vision, visual acuity, adaptation, and the perceptual consequences of retinal dysfunction.

Retina Photoreceptors - Related Videos

Research

JoVE Journal - Neuroscience
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Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

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Cited by 24 •

2015

We describe a protocol to monitor Ca2+ dynamics in the axon terminals of cone photoreceptors using an ex-vivo slice preparation of the mouse retina. This protocol allows comprehensive studies of cone Ca2+ signaling in an important mammalian model system, the mouse.

Research

JoVE Journal - Medicine

Subretinal Transplantation of MACS Purified Photoreceptor Precursor Cells into the Adult Mouse Retina

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Cited by 43 •

2014

Cell transplantation represents a strategy for the treatment of retinal degeneration characterized by photoreceptor loss. Here we describe a method for enrichment of transplantable photoreceptors and their subretinal grafting into adult mice.

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JoVE Journal - Neuroscience
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Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures

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Cited by 15 •

2014

Neural retina of a mouse aged 8 days is on top of a 4% gelatin block. After isolation of the photoreceptor layer (200 µm) by vibratome, the photoreceptors are seeded after mechanical and enzymatic dissociation for culture. The photoreceptor layer can be used for molecular, biochemical analyses or transplantation.

Research

JoVE Journal - Neuroscience
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Split Retina as an Improved Flatmount Preparation for Studying Inner Nuclear Layer Neurons in Vertebrate Retina

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2024

This work presents an alternative flatmount retina preparation in which the removal of photoreceptor cell bodies enables faster antibody diffusion and improved patch pipette access to inner retinal neurons for immunohistochemistry, in situ hybridization, and electrophysiology experiments.

Single-cell Suction Recordings from Mouse Cone Photoreceptors

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Cited by 9 •

2010

We will show how to record flash responses from single mouse cones using a suction electrode.

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