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Subretinal Transplantation of MACS Purified Photoreceptor Precursor Cells into the Adult Mouse Retina
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Subretinal Transplantation of MACS Purified Photoreceptor Precursor Cells into the Adult Mouse Retina

Subretinal Transplantation of MACS Purified Photoreceptor Precursor Cells into the Adult Mouse Retina

DOI:
10.3791/50932-v

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08:28 min

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February 22, 2014

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, , ,
1CRTD / DFG-Research Center for Regenerative Therapies Dresden,Technische Universität Dresden

Chapters

  • 00:05Title
  • 01:33Retina Dissociation
  • 03:21Cell Sorting Using Magnetic Associated Cell Sorting (MACS)
  • 05:07Transplantation of MAC-sorted Photoreceptor Precursor Cells Into the Mouse Retina
  • 06:39Results: Donor GFP+ Rod Photoreceptor Integration Into the Host Retina
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

Cell transplantation represents a strategy for the treatment of retinal degeneration characterized by photoreceptor loss. Here we describe a method for enrichment of transplantable photoreceptors and their subretinal grafting into adult mice.

Tags

Keywords: Subretinal TransplantationPhotoreceptor Precursor CellsMACSAdult Mouse RetinaVision ImpairmentBlindnessPhotoreceptor ReplacementCell IntegrationCell SortingCD73Rod Photoreceptor Precursors

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