Laser Excitation Method
Laser Excitation Method - Related Videos
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Synthesis of Gold Nanoparticle Integrated Photo-responsive Liposomes and Measurement of Their Microbubble Cavitation upon Pulse Laser Excitation
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Cited by 2 •
2016
This protocol describes a simple preparation method for gold nanoparticle integrated photo-responsive liposomes with the commercially available materials. It also shows how to measure the microbubble cavitation process of the synthesized liposomes upon the treatment of pulsed laser.
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Thermal Diffusivity and the Laser Flash Method
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2023
Source: Elise S.D. Buki, Danielle N. Beatty, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT The laser flash method (LFA) is a technique used to measure thermal diffusivity, a material specific property. Thermal diffusivity (α) is the ratio of how much heat is conducted relative to how much heat is stored in a material. It is related to thermal conductivity (), how much heat is transferred through a material due to a temperature...
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A Multimodal Imaging- and Stimulation-based Method of Evaluating Connectivity-related Brain Excitability in Patients with Epilepsy
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Cited by 5 •
2016
Resting-state functional-connectivity MRI has identified abnormalities in patients with a wide range of neuropsychiatric disorders, including epilepsy due to malformations of cortical development. Transcranial Magnetic Stimulation in combination with EEG can demonstrate that patients with epilepsy have cortical hyperexcitability in regions with abnormal connectivity.
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Corticospinal Excitability Modulation During Action Observation
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Cited by 22 •
2013
Single-pulse transcranial magnetic stimulation over the primary motor cortex, neuronavigation, and registration of electromyographic activity of hand muscles were used in this study to explore corticospinal excitability while participants were observing action sequences.
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Subsurface Defect Localization by Structured Heating Using Laser Projected Photothermal Thermography
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Cited by 7 •
2017
This method aims at locating vertical subsurface defects. Here, we couple a laser with a spatial light modulator and trigger its video input to heat a sample surface deterministically with two anti-phased modulated lines while acquiring highly resolved thermal images. The defect position is retrieved from evaluating thermal wave interference minima.
FAQs
Laser wavelength should match the absorption or excitation spectrum of the fluorophore, chromophore, or light-sensitive protein being studied. This spectral match determines whether the probe can absorb the incident photons and enter an excited state. In bioengineering experiments, selecting the laser this way connects the optical source to the specific biological component that must be measured or manipulated.
After absorbing photons, electrons in the probe move to higher energy states. As they relax, the probe can emit fluorescence or produce another optical response. Detecting that response converts the probe’s light-driven state change into an experimentally useful measurement. The resulting signal can therefore report on a biological probe or engineered system through optical readout.
Focusing and wavelength filtering serve different but complementary roles. Spatial focusing concentrates excitation at a selected location, while filtering helps isolate the emitted fluorescence or other optical response for detection. Together, these controls improve signal localization and experimental control, which is especially important when measurements must be associated with a particular region of a biological sample.
A basic workflow begins by identifying the fluorophore, chromophore, or light-sensitive protein, then choosing a laser that matches its absorption or excitation spectrum. The beam is focused onto the material or biological probe, and the resulting fluorescence or other optical response is collected with wavelength filtering. This sequence links probe selection, illumination, and optical readout.
Its applications include fluorescence microscopy, cellular imaging, biosensor development, and engineered biological systems. In these settings, controlled illumination can stimulate a selected probe and support optical readout or manipulation. These uses connect focused optical stimulation with measurements in cells, sensor designs, and engineered biological systems under study.
In bioengineering, the method can be applied wherever a fluorophore, chromophore, or light-sensitive protein serves as the optical interface. Matching the laser to that component enables excitation specific to the designed probe, while emitted fluorescence or another response supplies a measurable output. This makes the approach relevant to biological imaging and engineered sensing systems.