Micropipette Droplet Deposition
Micropipette Droplet Deposition - Related Videos
09:54
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
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Cited by 8 •
2015
Bacterial mechanosensitive channels can be used as mechanoelectrical transducers in biomolecular devices. Droplet interface bilayers (DIBs), cell-inspired building blocks to such devices, represent new platforms to incorporate and stimulate mechanosensitive channels. Here, we demonstrate a new micropipette-based method of forming DIBs, allowing the study of mechanosensitive channels under mechanical stimulation.
12:25
Counting Proteins in Single Cells with Addressable Droplet Microarrays
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Cited by 2 •
2018
Here we present addressable droplet microarrays (ADMs), a droplet array based method able to determine absolute protein abundance in single cells. We demonstrate the capability of ADMs to characterize the heterogeneity in expression of the tumor suppressor protein p53 in a human cancer cell line.
02:33
Phase Transitions: Sublimation and Deposition
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2020
Some solids can transition directly into the gaseous state, bypassing the liquid state, via a process known as sublimation. At room temperature and standard pressure, a piece of dry ice (solid CO2) sublimes, appearing to gradually disappear without ever forming any liquid. Snow and ice sublimate at temperatures below the melting point of water, a slow process that may be accelerated by winds and the reduced atmospheric pressures at high altitudes. When solid iodine is warmed, the solid sublimes...
10:31
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
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Cited by 30 •
2012
Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.
13:10
Title Cell Encapsulation by Droplets
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Cited by 4 •
2007
FAQs
Surface tension helps determine whether a liquid forms a stable droplet, spreads across the receiving surface, or releases from the micropipette. Fluid properties also influence the droplet’s behavior during dispensing and after contact. These factors matter because they affect placement, shape, and consistency, which in turn influence the reproducibility of microscale bioengineering experiments.
Controlled movement positions the micropipette accurately, while contact with the target location supports predictable droplet release. Together, these actions help limit variation in where the liquid is deposited and how it spreads. Consistent positioning and contact are especially important when droplets contain cells, biomaterials, or reagents intended for localized patterns or defined experimental regions.
The technique supports precise handling of small liquid volumes, so experiments can be performed without requiring large amounts of cells, biomaterials, or biological reagents. This low sample requirement complements accurate placement and helps researchers create localized experimental conditions. As a result, the method is useful when conserving valuable biological materials while maintaining microscale control.
Reproducibility comes from combining measured aspiration, controlled dispensing, and deliberate placement at a defined location. The resulting droplets can establish comparable liquid or material arrangements across experiments. Because deposition occurs at a microscale and uses controlled volumes, researchers can more consistently prepare localized assays, cell patterns, and biomaterial configurations for bioengineering studies.
A typical workflow begins by aspirating a measured volume into the micropipette. The pipette is then moved to the selected surface or defined location, positioned for deposition, and brought into controlled contact as the liquid is dispensed. The deposited droplet is subsequently used in the intended pattern, assay, biomaterial arrangement, or tissue-engineering workflow.
Micropipette droplet deposition can support cell patterning, localized biological assays, biomaterial arrangement, and tissue-engineering workflows. Each application uses controlled placement to organize biological materials or reagents in specific microscale locations. This makes the technique relevant to engineered biological systems where spatial arrangement and localized experimental conditions contribute to the study or construction of bioengineered structures.
The approach can produce controlled droplet placement, localized reagent or cell distributions, and organized biomaterial arrangements. These outcomes help researchers create defined microscale environments rather than applying materials broadly across a surface. Its combination of precise handling and low sample requirements supports systematic experimentation and the development of engineered biological systems.