Calcite Crystal Formation

Calcite crystal formation is the nucleation and growth of calcium carbonate crystals in the calcite mineral form, a process important in both geological systems and biological materials. Crystals develop when dissolved calcium and carbonate ions reach supersaturation, allowing nuclei to form and grow as ions attach to ordered surfaces; organisms can regulate this process through organic matrices, proteins, pH, and ion concentrations. In biology, calcite biomineralization contributes to shells, protective structures, and skeletal elements in organisms such as mollusks and some algae. Studying these mechanisms helps explain mineralized tissue development, environmental adaptation, and how organisms control crystal structure and morphology.

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JoVE EoE - Bacterial Growth and Techniques

Microbiologically Induced Calcite Precipitation by Sporosarcina pasteurii

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2025

Source: Bhaduri, S., et al., Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii. J. Vis. Exp. (2016)This video demonstrates the method of enriching Sporosarcina pasteurii with urea and calcium chloride to trigger urease activity, leading to the formation of visible calcite crystals through Microbiologically Induced Calcite Precipitation, or MICP.

Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii

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2016

Protocols for microbiologically induced calcite precipitation (MICP) using the bacterium Sporosarcina pasteurii are presented here. The precipitated calcium carbonate was characterized through optical microscopy and scanning electron microscopy (SEM). It is also shown that exposure to MICP increases the compressive strength of sponge.

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JoVE Science Education - Environmental Sciences
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Physical Properties Of Minerals I: Crystals and Cleavage

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2023

Source: Laboratory of Alan Lester - University of Colorado Boulder The physical properties of minerals comprise various measurable and discernible attributes, including color, streak, magnetic properties, hardness, crystal growth form, and crystal cleavage. Each of these properties are mineral-specific, and they are fundamentally related to a particular mineral’s chemical make-up and atomic structure. This experiment examines two properties that stem primarily from symmetric repetition of...

Protein Crystallization

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2023

Protein crystallization, obtaining a solid lattice of biomolecules, elucidates protein structure and enables the study of protein function. Crystallization involves drying purified protein under a combination of many factors, including pH, temperature, ionic strength, and protein concentration. Once crystals are obtained, the protein structure can be elucidated by x-ray diffraction and computation of an electron density model. This video introduces protein crystallization and shows a general...

Crystal Violet Staining: A Technique to Visualize Drug-Resistant Cancer Cells Using Colony Formation Assays

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2025

In this video, we demonstrate a cell viability assay using a crystal violet staining procedure for chemotherapeutic drug-resistant cancer cells.

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