Fungal Microbiota
Fungal Microbiota - Related Videos
08:52
Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
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Cited by 22 •
2013
We describe methods for live-cell video microscopy of Candida albicans phagocytosis by macrophages. These methods enable stage-specific analysis of macrophage migration, recognition, engulfment and phagosome maturation and reveal novel aspects of phagocytosis.
07:31
Microbial and Fungal Diversity - Student Protocol
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2019
Examining Microbial Colony Structures ExpandTo begin, collect your allocated culture plates and then remove the Parafilm and lid from one of them. Place the uncovered plate under a dissecting microscope and then observe the individual communities within each quadrant of the plate. As the colonies are identified, fill out the observed colony properties in the appropriate column in the table. Click Here to download Table 1 Then, repeat the observations for each of the other two bacterial species.
05:06
Microbial and Fungal Diversity - Concepts
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2019
Bacteria and fungi are two highly diverse groups of organisms that can have significant beneficial or detrimental impacts on human health. For this reason, it is important to understand and distinguish between individual species of these groups. As you will recall, biological taxonomists group organisms based on their phylogenetic relatedness. The three domains of life, Bacteria, Archaea, and Eukaryota segregate life into three distinct groupings. While all bacteria fall within the domain...
01:15
Assessing the Role of Gut Microbiota in Modulating Hepatitis B Infection in Mice
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2026
Take a control mouse with a normal gut microbiota and an experimental mouse with depleted gut microbiota. The depletion compromises the integrity of the gut barrier.This enables bacteria to translocate through the blood vessel into the liver tissue.Inject both mice with the hepatitis B virus or HBV plasmid to induce an HBV infection in the liver tissue.In the control mouse, the T cell detects an infected liver cell through the interaction of its receptors with viral antigens, and produces...
04:14
Establishing a Fungal-Bacterial Co-culture for Biofilm Formation
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2026
Source: Miquel Guennoc, C., et al. New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy. J. Vis. Exp. (2017)This video demonstrates the preparation of a fungal culture on treated cellophane membranes to support colony formation and radial growth. It then shows the cultivation and preparation of soil bacterial cells for interaction assays. Finally, it illustrates how bacterial cells adhere to the fungal colony...
FAQs
Local establishment depends on several linked processes: fungi compete for nutrients, associate physically with surrounding cells or surfaces, and release enzymes and metabolites that alter their immediate environment. These activities help determine which fungi persist and how strongly they influence nearby organisms. Their effects therefore reflect both resource availability and interactions within the surrounding biological community.
Secreted enzymes and metabolites allow fungi to modify available nutrients and affect neighboring organisms without requiring direct cellular contact. They can contribute to nutrient use, physical association, and communication within a microbial or host environment. Examining these products helps connect fungal presence with biological function rather than treating community composition alone as the primary outcome.
Fungal communities can influence, and respond to, signaling pathways involving their hosts or other microbes. These interactions help shape local biological conditions and may affect immunity, disease-related processes, plant health, or microbial community stability. Studying signaling alongside fungal abundance provides a more functional view of how community members contribute to host or ecosystem behavior.
Culture-based methods, microscopy, and sequencing provide complementary views of fungal communities. Culture can support examination of organisms that grow under selected conditions, microscopy reveals local presence and physical associations, and sequencing helps characterize community composition. Comparing results across methods can support investigations of how treatments or environmental changes alter fungal abundance and community structure.
A study can begin by selecting a defined environment, such as soil, a plant, an animal, or the human body, then examining its fungal community with culture, microscopy, sequencing, or a combination of these approaches. Researchers can compare samples exposed to different conditions or treatments and relate changes in composition or abundance to biological function.
These studies can examine nutrient cycling, plant health, host immunity, disease, and microbial community stability. In soil, the focus may be how fungal communities contribute to ecological function; in hosts or plants, it may involve associations with health or disease. Measurements of community composition and abundance help connect environmental change or treatment to these broader outcomes.