Transgenic Plants
Transgenic Plants - Related Videos
02:50
Transgenic Plants
0 Views •
2021
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms. The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
05:09
Binary Bacterial Artificial Chromosome Vector Based Gene Transformation in Arabidopsis Plants: A Technique to Generate Transgenic Plants With Single-Copy Insertion
0 Views •
2025
In this video, we demonstrate generation of transgenic Arabidopsis plants expressing stable transgene through Agrobacterium-mediated binary bacterial artificial chromosome-based gene transformation.
13:37
Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
0 Views •
Cited by 7 •
2011
We demonstrate how hairy root composite plants can be used to study plant-rhizobium interactions and nodulation in the difficult-to-transform species Medicago truncatula.
12:08
Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
0 Views •
Cited by 4 •
2018
Using a pBIBAC-GW binary vector makes generating transgenic plants with intact single-copy insertions, an easy process. Here, a series of protocols is presented that guide the reader through the process of generating transgenic Arabidopsis plants, and testing the plants for intactness and copy number of the inserts.
10:22
Transmitting Plant Viruses Using Whiteflies
0 Views •
Cited by 41 •
2013
Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...
FAQs
These approaches provide two routes for introducing a gene of interest into plant cells. Agrobacterium-mediated transformation uses Agrobacterium to deliver the genetic material, whereas a gene gun physically delivers it into cells. The source material identifies both as standard transformation methods, after which researchers select modified cells and regenerate them into complete plants through tissue culture.
Gene introduction does not by itself produce a complete modified plant. Researchers first select cells that have acquired the introduced gene, then use tissue culture to regenerate those cells into whole plants. This sequence connects cellular transformation with a recoverable plant, allowing scientists to examine the resulting trait or study how the introduced gene functions.
An introduced gene can produce a characteristic such as insect resistance, herbicide tolerance, improved nutritional value, or resistance to environmental stress. The resulting plant also provides a system for examining gene expression, plant development, and genome function. Comparing these outcomes helps biology researchers connect a particular gene with observable plant characteristics or developmental processes.
A typical workflow begins by introducing a gene of interest into plant cells with Agrobacterium-mediated transformation or a gene gun. Scientists then select the transformed cells and regenerate them through tissue culture into whole plants. The regenerated plants can subsequently be examined for the intended useful trait or used to investigate gene expression and plant biology.
They are used when researchers aim to develop plants with useful characteristics, including insect resistance, herbicide tolerance, improved nutritional value, or resistance to environmental stress. These traits connect genetic research with crop improvement and sustainable agriculture. The approach can therefore support both the study of plant genomes and efforts to produce plants with agricultural value.
In biological research, these plants help scientists investigate gene expression, plant development, and genome function. Beyond basic research, they contribute to crop improvement, sustainable agriculture, and pharmaceutical production. Their value comes from linking an introduced gene to a measurable plant trait or biological process, creating opportunities to study mechanisms while developing useful products or characteristics.