Surgical Dissection
Surgical Dissection - Related Videos
08:49
Murine Prostate Micro-dissection and Surgical Castration
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Cited by 23 •
2016
This manuscript describes the protocols for prostate micro-dissection and surgical castration in the laboratory mouse. We also depict representative results produced by these protocols. Finally, we discuss the advantages and utilization of these protocols.
02:37
Dissection of Palate Tissue from Adult Murine Model: A Surgical Procedure to Harvest Hard Palate and Soft Palate from Oral Cavity of Adult Mouse
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2025
This video demonstrates a surgical procedure to dissect the palate tissue from a murine model.
07:40
Dissection of the Adult Zebrafish Kidney
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Cited by 38 •
2011
The zebrafish kidney is home to both renal and hematopoietic adult stem/progenitor cells, and represents an outstanding opportunity to study these cell types and their progeny in a vertebrate model organism. Here, we demonstrate a detailed dissection procedure that enables the researcher to identify and surgically remove the adult zebrafish kidney, which can be used for applications such as cell isolation, transplantation, and expression studies of kidney and/or blood cell populations.
07:25
Open Surgical Resection of Cerebral Arteriovenous Malformations
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2025
Source: Rennert, R. C., et al. Comprehensive Endovascular and Open Surgical Management of Cerebral Arteriovenous Malformations. J. Vis. Exp. (2017).This video demonstrates the open surgical resection of a cerebral arteriovenous malformation (AVM) in a human brain. The procedure involves creating a bone flap, accessing the subarachnoid space, identifying and occluding feeding arteries and draining veins, and carefully removing the AVM. Intraoperative angiography confirms complete resection,...
03:54
Zebrafish Brain Dissection: A Technique of Fish Neurobiology
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2023
This video describes the technique of microdissection of the brain from an adult zebrafish. This method helps in obtaining whole brain derived neuorospheres that can be studied further.
FAQs
Preserving tissue architecture keeps the spatial relationships among tumor cells, adjacent normal tissue, lymph nodes, and surrounding microenvironment intact. That context helps investigators evaluate local invasion and clinically relevant margins rather than examining isolated cells alone. It also supports histopathological interpretation and can improve the reliability of later molecular or cellular analyses.
The approach separates specimens according to their anatomical and pathological relevance, including the tumor, nearby normal tissue, and lymph nodes when appropriate. This organized sampling creates direct comparison groups within the same investigation. Such comparisons help reveal disease-associated changes, evaluate local spread, and assess whether treatment responses differ between malignant and nonmalignant tissues.
Specimen value depends on whether the dissection preserves relevant architecture and retains the tissues needed for the planned analysis. Including tumor material alongside adjacent normal tissue, lymph nodes, or clinically relevant margins can broaden interpretation. Careful separation also reduces loss of information about tumor boundaries, local invasion, metastasis, and interactions with the surrounding microenvironment.
A typical workflow identifies the tumor and relevant neighboring structures, exposes the selected region, and removes tumor, adjacent normal tissue, or lymph-node specimens while maintaining useful tissue architecture. The collected material is then organized for downstream histopathological, molecular, or cellular analysis. The specific specimens selected should match the research question, such as invasion or treatment response.
Dissected specimens can support histopathological examination, molecular analysis, and cellular analysis. Histopathology can characterize tissue changes and tumor relationships, while molecular or cellular studies can investigate disease-associated features in selected samples. Together, these readouts may contribute to tumor classification, biomarker research, treatment-response assessment, and evaluation of interactions between tumors and their surrounding microenvironment.
Carefully collected tumor and related tissues provide higher-quality starting material for developing cancer models and identifying biomarkers. Comparing tumor specimens with adjacent normal tissue or lymph nodes can help connect candidate findings to disease-associated changes. The resulting samples also support studies of local invasion, metastasis, treatment response, and tumor interactions with the surrounding microenvironment.