Cartridge-based Technique
Cartridge-based Technique - Related Videos
08:04
Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
0 Views •
Cited by 133 •
2016
We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.
03:31
A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E
0 Views •
2025
This video demonstrates a protocol for detecting serum-specific immunoglobulin E using a microfluidic cartridge-based chemiluminescence system. Serum IgEs interact with distinct allergens immobilized in the reaction zone of the cartridge, which are subsequently detected with peroxidase-conjugated anti-IgE antibodies and a chemiluminescent substrate, allowing simultaneous detection of multiple allergen-specific IgEs.
05:05
Technique of Conjunctival Biopsy and Direct Immunofluorescence for Diagnosing Mucous Membrane Pemphigoid
0 Views •
2025
Here, we present a protocol for harvesting and processing conjunctival biopsy to identify mucous membrane pemphigoid.
04:14
Lab Techniques - Concepts
0 Views •
2020
Measurement Accuracy The ability to repeat an experiment and get the same results, or reproducibility, is essential in scientific research. However, it’s impossible to repeat an experiment if you don’t know how you did it. Thus, scientists keep detailed records of their experiments in lab notebooks. These records include important information like the amount of each material used and descriptions of each step of the procedure. Equipment, like balances and volumetric or graduated glassware, is...
08:12
Zebrafish Microinjection Techniques
0 Views •
2023
One of the major advantages to working with zebrafish (Danio rerio) is that their genetics can be easily manipulated by microinjection of early stage embryos. Using this technique, solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is achieved either through direct injection into the blastomere, or via natural cytoplasmic movements that occur after a...
FAQs
Selectivity depends on how individual sample components interact with the cartridge’s internal material. A sorbent can retain some compounds while allowing others to pass, whereas a reagent or reactive material can alter a selected compound during processing. These different interactions let chemists remove unwanted components, isolate target analytes, or promote a controlled chemical transformation.
Changing the solvent can modify the interaction between the target analyte and the cartridge material. Under one condition, the analyte may remain associated with the internal phase; under another, it can be released for collection or undergo a desired transformation. Controlled solvent changes therefore help determine whether the cartridge functions primarily for separation, recovery, or reaction.
Controlled flow determines how consistently the sample contacts the cartridge contents. Consistent contact supports reproducible retention, removal, separation, or transformation across samples, while uncontrolled handling can make processing less uniform. In chemistry laboratories, this control is especially valuable when the method must produce comparable sample preparation or analytical results across repeated runs.
A typical workflow places the sample into a prepared cartridge, passes it through the internal sorbent, reagent, or reactive material under controlled flow, and then changes the solvent or operating conditions as needed. The resulting fractions can contain removed components, retained material, or released target analyte. This sequence supports sample preparation, purification, extraction, and chemical analysis.
Chemists may choose this format when they need a modular workflow with reduced sample handling and consistent processing. Prepacked, often disposable cartridges can support repeated preparation, purification, extraction, or analysis while limiting opportunities for cross-contamination. The approach is also useful when laboratories want to connect sample processing with higher-throughput or automated operation.
In analytical chemistry, cartridges can help prepare samples before measurement by separating or removing components that interfere with analysis, or by recovering a target analyte under changed conditions. Their modular format supports reproducible workflows, while disposable designs can reduce cross-contamination. These features make the technique relevant to research and routine laboratory processing where consistency and throughput matter.