Intestinal Transit
Intestinal Transit - Related Videos
08:15
Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids
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Cited by 7 •
2019
This protocol describes how to mimic suckling-to-weaning transition in vitro using mouse late fetal intestinal organoids cultured for 30 days.
02:31
Phase Transitions
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2020
Whether solid, liquid, or gas, a substance's state depends on the order and arrangement of its particles (atoms, molecules, or ions). Particles in the solid pack closely together, generally in a pattern. The particles vibrate about their fixed positions but do not move or squeeze past their neighbors. In liquids, although the particles are closely spaced, they are randomly arranged. The position of the particles are not fixed—that is, they are free to move past their neighbors to occupy...
Live-Cell Imaging and Single-Cell Tracking to Monitor Clonal Dynamics and Fate Transitions in Human and Murine Intestinal Organoids
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2026
We describe confocal imaging and tracking pipelines applied to two reporter strategies: mosaic organoids for resolving individual cell behavior and fate reporter organoids for monitoring cell type transitions in human and murine intestinal organoids.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
01:58
Cooperative Allosteric Transitions
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2020
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
FAQs
Intestinal smooth-muscle contractions perform two related tasks: they mix luminal contents and propel them toward the colon. Mixing helps expose ingested material to digestive processes and supports nutrient absorption, while coordinated propulsion advances the contents through the gastrointestinal tract. The balance between these actions influences how efficiently material progresses and how long it remains available for intestinal processing.
The enteric nervous system regulates the activity of intestinal smooth muscle, helping coordinate contractions along the gastrointestinal tract. This coordination determines whether contents are primarily mixed or moved forward. Because transit depends on organized muscular activity rather than isolated contractions, changes affecting this regulatory relationship can contribute to altered gastrointestinal motility and abnormal passage of intestinal contents.
Fluid content and meal composition are important influences on how luminal contents move through the intestine. They can affect the material being mixed and propelled by smooth-muscle contractions, thereby influencing passage through the gastrointestinal tract. Considering these factors helps explain why transit is dynamic and why intestinal movement may vary with the characteristics of an ingested meal.
Measuring intestinal transit provides information about gastrointestinal motility, particularly whether material is passing through the tract more slowly or more rapidly than expected. This information can help clinicians evaluate abnormal movement patterns and distinguish delayed passage associated with constipation from accelerated passage associated with diarrhea. The results contribute to a more focused assessment of digestive function.
Clinicians may assess intestinal transit when symptoms suggest that gastrointestinal contents are moving at an abnormal rate. The assessment is especially relevant when distinguishing delayed passage, such as constipation, from accelerated passage, such as diarrhea. Transit information can support diagnostic evaluation, clarify whether motility is involved, and help guide decisions about subsequent treatment.
Transit findings can guide treatment decisions by identifying whether a patient’s digestive symptoms are associated with delayed or accelerated passage. Beyond individual care, these measurements improve understanding of disorders that affect normal gastrointestinal motility. They therefore serve both a practical clinical role in evaluation and management and a research role in studying abnormal digestive function.