Crispr Genome Editing
Crispr Genome Editing - Related Videos
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
02:58
CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
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2025
This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide substitution.
01:28
CRISPR/Cas9 Genome Editing
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2025
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
07:56
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Cited by 5 •
2019
CRISPR-Cas is a powerful technology to engineer the complex genomes of plants and animals. Here, we detail a protocol to efficiently edit the human genome using different Cas endonucleases. We highlight important considerations and design parameters to optimize editing efficiency.
05:12
All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System
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2025
In this video, we demonstrate all-in-one CRISPR-Cas9 based genome editing in cultured cells where Cas9 and sgRNA are provided as a single plasmid construct to the cells. The CRISPR-Cas9 system and desired gene to be inserted was introduced in cells through electroporation technique to facilitate successful gene editing.
FAQs
The PAM, or protospacer adjacent motif, provides a required sequence landmark next to the DNA region recognized by the guide RNA. A matching guide sequence is therefore not sufficient by itself: the appropriate PAM must also be present for nuclease targeting. This requirement constrains which genetic sites are eligible and where editing can occur.
Guide RNA and Cas9 contribute different parts of the targeting process. The guide RNA supplies sequence complementarity to the selected DNA, while Cas9 acts as the CRISPR-associated nuclease that cuts the DNA. Their coordination connects sequence recognition with strand cleavage, allowing researchers to direct a break toward a locus chosen for genetic investigation or modification.
The repair pathway strongly influences the resulting genetic change. Non-homologous end joining reconnects broken DNA ends and can be used when the goal is to disrupt a gene. Homology-directed repair instead uses a supplied template, supporting correction or other planned sequence modifications. Selecting the pathway therefore links the DNA break to the intended genetic outcome.
A CRISPR genome editing workflow begins by identifying a genetic sequence of interest and checking for a nearby PAM. Researchers then select a complementary guide RNA and compatible nuclease, define whether the goal is disruption, correction, or modification, and determine whether repair should rely on end joining or a supplied template. The planned repair outcome guides the experimental design.
By deliberately disrupting, correcting, or modifying selected genes, researchers can examine how those genes contribute to biological traits or disease-related processes. In genetics, the resulting changes support functional studies and disease modeling. Comparing the intended genetic alteration with its observed consequences helps connect a DNA sequence to gene function or a disease phenotype.
The same ability to make targeted genetic changes supports several areas of genetics. Researchers apply it to disease modeling, investigate potential treatments for genetic disease, and pursue crop improvement. These applications use controlled changes in DNA to test biological hypotheses, explore useful traits, or evaluate emerging therapeutic strategies.