Faire Assay
Faire Assay - Related Videos
14:32
Using Visual and Narrative Methods to Achieve Fair Process in Clinical Care
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Cited by 26 •
2011
This paper illustrates an innovative visual approach (photovoice or photo-elicitation) to achieve fair process in clinical care for patients living with chronic health conditions, illuminate gaps in clinical knowledge, forge better therapeutic relationships, and identify patient-centered goals and possibilities for healing.
01:22
Fair Value Principle
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2025
The fair value principle in accounting mandates measuring and reporting assets and liabilities at their current market value rather than their historical purchase cost. This principle aims to present a more accurate and timely reflection of an entity’s financial position, particularly in environments characterized by fluctuating market conditions. Fair value accounting is especially relevant for financial instruments and other assets whose values are sensitive to market dynamics.Under fair...
01:29
Cost Basis vs. Fair Value Accounting
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2025
Cost basis and fair value accounting represent two contrasting methods for valuing assets, each with distinct implications for financial reporting and decision-making. These methods not only influence how companies report their financial position but also affect investor perceptions and regulatory compliance.Cost basis accounting relies on the historical cost principle, meaning assets are recorded at their acquisition price and remain unchanged over time unless impaired. This approach is valued...
08:12
The TUNEL Assay
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2023
One of the hallmarks of apoptosis is the nuclear DNA fragmentation by nucleases. These enzymes are activated by caspases, the family of proteins that execute the cell death program. TUNEL assay is a method that takes advantage of this feature to detect apoptotic cells. In this assay, an enzyme called terminal deoxynucleotidyl transferase catalyzes the addition of dUTP nucleotides to the free 3’ ends of fragmented DNA. By using dUTPs that are labeled with chemical tags that can produce...
09:12
Aortic Ring Assay
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Cited by 42 •
2009
Angiogenesis, the sprouting of blood vessels from pre-existing vasculature, is associated with both natural and pathological processes. Here we demonstrate an aortic ring assay that allows angiogenic potentiators and inhibitors to be directly added to aortic rings in culture. Sprouting and neovessel outgrowth can be determined by inspecting the aortic rings over a period of 6-12 days.
FAQs
Formaldehyde preserves interactions between DNA and associated proteins, including nucleosomes, before the chromatin is processed. This crosslinking step allows the extraction procedure to distinguish DNA that remains tightly associated with chromatin proteins from DNA that is less associated. The resulting partitioning provides the basis for enriching sequences from regions with greater chromatin accessibility.
During phenol-chloroform extraction, DNA that is not tightly associated with chromatin proteins preferentially partitions into the aqueous phase. Because nucleosome-associated DNA behaves differently after crosslinking and processing, the aqueous fraction becomes enriched for sequences from less densely protein-associated regions. Researchers can then examine this fraction to identify candidate regulatory DNA.
Nucleosomes are among the chromatin proteins crosslinked to DNA during the assay, so their association influences which sequences remain linked to chromatin material during extraction. Regions with stronger nucleosome association are less represented in the enriched aqueous DNA, whereas less tightly associated sequences are preferentially recovered. This relationship connects the assay signal to chromatin accessibility and potential regulatory activity.
The workflow begins by crosslinking DNA and chromatin proteins with formaldehyde, followed by cell lysis and sonication. The processed material then undergoes phenol-chloroform extraction, which separates DNA according to its association with chromatin proteins. DNA recovered from the aqueous phase is enriched and prepared for downstream analysis by PCR or sequencing.
PCR is useful when researchers want to examine selected genomic regions, such as a suspected promoter or enhancer. Sequencing provides a broader view of enriched DNA across many genomic locations and can support genome-wide mapping of accessible regulatory elements. The choice therefore depends on whether the study focuses on targeted validation or wider discovery of regulatory sequences.
FAIRE assay data can help connect chromatin accessibility with genetic regulation by locating promoters, enhancers, and other candidate control regions. In genetics, these maps support investigations of developmental regulation, disease-associated variants, and genome-wide control of transcription. Enriched regions provide genomic locations for examining how regulatory DNA may contribute to differences in gene expression.