Cd14+ Monocytes
Cd14+ Monocytes - Related Videos
11:52
Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
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Cited by 6 •
2023
Osteoclasts are key bone-resorbing cells in the body. This protocol describes a reliable method for the in vitro differentiation of osteoclasts from human peripheral blood monocytes. This method can be used as an important tool to further understand osteoclast biology in homeostasis and in diseases.
08:31
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
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Cited by 19 •
2016
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.
02:46
An In Vitro Assay for Evaluating the Immunomodulatory Impact of Monocytes on Leukocyte Proliferation
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2025
This video demonstrates an in vitro assay to explore the immunomodulatory influence of mesenchymal stem cells (MSCs) on effector T cell proliferation. The assay includes co-culturing MSCs with PBMCs to induce the monocyte population to acquire an immunomodulatory phenotype, leading to the secretion of interleukin-10 (IL-10). Following incubation with activated CD4+ T cells, the induced monocytes effectively suppress T cell proliferation.
11:34
A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes
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Cited by 1 •
2025
We describe a procedure to assess the capacity for pharmacologic agents to generate tolerogenic dendritic cells from naïve monocyte-derived dendritic cells in vitro and validate their potency by autologous regulatory T cell generation.
06:28
A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood
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Cited by 13 •
2016
Monocytes are integral components of the human innate immune system that rely on glycolytic metabolism when activated. We describe a flow cytometry protocol to measure glucose transporter expression and glucose uptake by total monocytes and monocyte subpopulations in fresh whole blood.
FAQs
Recognition begins when CD14 binds bacterial lipopolysaccharide (LPS) together with LPS-binding protein. CD14 then facilitates signaling through the Toll-like receptor 4 (TLR4) complex. This signaling changes gene expression and promotes cytokine production and antimicrobial activity, linking detection of a bacterial component to an innate immune response.
After entering tissues, these cells can differentiate into macrophages or dendritic cells. That transition changes the type of immune cell present at the tissue site and connects circulating monocytes with more specialized cellular participants in inflammation and antimicrobial defense. Tracking this potential is therefore important when interpreting monocyte responses beyond the bloodstream.
CD14 provides a surface-based way to identify the monocyte population, but it does not by itself describe every state those cells may occupy. Researchers therefore combine CD14 identification with flow-cytometric assessment of subsets, abundance, activation, and maturation. This separates cell identification from interpretation of functional or developmental status.
Flow cytometry is used to distinguish CD14+ monocyte subsets and to track how their abundance, activation, and maturation vary in a sample. The resulting profile can show whether an immune response is associated with shifts in population size or cellular state. It therefore provides a comparative framework for characterizing host responses to infection and inflammation.
In infection research, these analyses help characterize how the host responds to inflammatory or pathogen-associated signals. Investigators can examine the abundance and state of CD14+ monocyte populations while considering their potential tissue differentiation. The approach is relevant to studying inflammation, sepsis, and pathogen-associated immune dysfunction, rather than focusing only on the invading organism.
Comparing subset abundance, activation, and maturation gives researchers several complementary indicators of immune status. In studies of sepsis or inflammatory disease, these measurements can help describe whether monocyte populations are changing alongside the host response. The data support characterization of disease-associated inflammation and immune dysfunction, although they represent cellular patterns rather than a complete explanation of disease.