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Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

DOI:
10.3791/64698-v

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11:52 min

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January 27, 2023

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, , ,
1School of Infection & Immunity,University of Glasgow

Chapters

  • 00:04Introduction
  • 00:56Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Whole Blood
  • 02:34Enrichment of CD14+ Monocytes from PBMCs
  • 04:20Osteoclast Differentiation In Vitro
  • 05:46TRAP Staining for Osteoclasts
  • 07:10Actin Ring Fluorescent Staining
  • 09:40Results: Differentiation of Osteoclasts and Assessing the Function of Mature Osteoclasts
  • 11:12Conclusion

Summary

Automatic Translation

Automatic Translation

Osteoclasts are key bone-resorbing cells in the body. This protocol describes a reliable method for the in vitro differentiation of osteoclasts from human peripheral blood monocytes. This method can be used as an important tool to further understand osteoclast biology in homeostasis and in diseases.

Tags

Keywords: Osteoclast DifferentiationPBMC IsolationCD14+ Monocyte EnrichmentIn Vitro Osteoclast GenerationCytokine StimulationBone ResorptionArthritisTherapeutic Screening

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