Cell Death Assay
Cell Death Assay - Related Videos
04:25
Neuronal Death Assay to Evaluate Cell Death in Ex Vivo Epilepsy Model
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2025
This video demonstrates the technique of assessing neuronal death in rhinal cortex-hippocampus organotypic slices. Neuronal death occurs via evolving epileptic seizures — resembling in vivo epilepsy — and is detected through dual-staining of the dead cells using propidium iodide and immunofluorescence.
07:35
A β-glucuronidase (GUS) Based Cell Death Assay
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Cited by 5 •
2011
Programmed cell death assays commonly used in mammalian systems such as DNA laddering or TUNEL assays, are often difficult to reproduce in plants. In combination with a GUS reporter system, we propose a rapid, plant based transient assay to analyze the potential death properties of specific genes.
10:06
An Introduction to Cell Death
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2023
Necrosis, apoptosis, and autophagic cell death are all manners in which cells can die, and these mechanisms can be induced by different stimuli, such as cell injury, low nutrient levels, or signaling proteins. Whereas necrosis is considered to be an “accidental” or unexpected form of cell death, evidence exists that apoptosis and autophagy are both programmed and “planned” by cells.In this introductory video, JoVE highlights key discoveries pertaining to cell death, including recent work done...
11:48
Assay for Cell Death: Chromium Release Assay of Cytotoxic Ability
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2023
Source: Frances V. Sjaastad1,2, Whitney Swanson2,3, and Thomas S. Griffith1,2,3,4 1 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Department of Urology, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 One of the main functions of the cells of the immune system is to remove target cells...
01:30
TUNEL Assay for Detecting Apoptotic Cell Death in a Brain Slice
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2025
To detect apoptotic cell death using the TUNEL assay, treat the brain slice with the enzyme proteinase-K.
FAQs
Different readouts report different cellular changes rather than interchangeable measures. A membrane-integrity signal addresses whether the plasma membrane remains intact, whereas reduced metabolic activity, DNA fragmentation, or apoptosis-associated proteins reflect other aspects of cellular injury or death. Consequently, discordant results may still be informative, showing that assays examine distinct features of the process.
To distinguish apoptosis from necrosis, researchers compare signals associated with each process instead of relying on a single measurement. DNA fragmentation and activation of apoptosis-associated proteins can provide evidence relevant to apoptosis, while loss of plasma membrane integrity indicates cellular damage or death. Comparing these patterns helps clarify the mode of cytotoxicity.
Microscopy adds spatial and visual information to a cell death assay. Fluorescent dyes can reveal signals in individual cells, while microscopy allows researchers to examine where those signals occur and relate them to cellular or tissue context. This complements enzyme-based signals and other fluorescence measurements when the distribution of affected cells is scientifically important.
In infection studies, cell death assays can separate damage caused by a pathogen from effects associated with an immune response. Measuring host-cell injury can reveal how infection alters tissues, while testing immune-cell activity can show whether those cells induce or prevent target-cell death. These results help connect cellular outcomes with disease mechanisms and tissue damage.
Selection starts with the biological question: researchers may need to detect death, quantify its extent, or distinguish among death states. They can then choose a compatible readout, such as a fluorescent dye, enzyme-based signal, or microscopy, and interpret the result according to the feature measured. This approach keeps conclusions tied to the relevant cellular process.
Cell death assays can evaluate potential antimicrobial or immunomodulatory treatments by showing whether an intervention changes cellular injury or death. In an infection model, the readout may indicate whether a candidate affects pathogen-associated host damage or modifies immune-cell effects. Comparing assay results across treatment conditions helps characterize the direction and nature of the cellular response.