Mdck Cells
Mdck Cells - Related Videos
12:09
Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
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Cited by 28 •
2017
Influenza neutralizing antibodies correlate with protection of influenza infections. Microneutralization assays measure neutralizing antibodies in human sera and are often used for influenza human serology. We describe a microneutralization assay using MDCK-SIAT1 cells to measure neutralizing antibody titers to contemporary 3C.2a and 3C.3a A(H3N2) viruses following influenza vaccination or infection.
09:38
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
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Cited by 5 •
2011
This article details the procedures involved in overexpression and analysis of small GTPases in polarized epithelial cells using microinjection technique.
03:09
Determining Optimal Target Cell Density Using a Luciferase-Based Pseudovirus Infection Assay
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2026
Source: Chang, X., et al. Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity. J. Vis. Exp. (2021)This video demonstrates a luciferase-based pseudovirus titration assay to determine the optimal target cell density for maximum infection. By comparing luminescence across cell densities, the method ensures high assay sensitivity and reproducibility for downstream applications.
03:34
Assessing Caspase-Mediated Cleavage of Viral and Host Proteins in Virus-Infected Cells
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2025
The video demonstrates a method to assess caspase-mediated protein cleavage in virus-infected cells. Post-viral infection, caspases get activated and degrade various host cellular proteins and viral nucleoproteins while specific caspase inhibitors prevent this degradation, as confirmed by western blotting.
11:31
Generation of Recombinant Influenza Virus from Plasmid DNA
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Cited by 129 •
2010
Rescue of influenza A viruses from plasmid DNA is a basic and essential experimental technique that allows influenza researchers to generate recombinant viruses to study multiple aspects in the biology of influenza virus, and to be used as potential vectors or vaccines.
FAQs
Confluent MDCK monolayers develop separate apical and basolateral surfaces, enabling researchers to examine whether viral entry, transport, or release differs by side of the epithelial barrier. This organization connects infection mechanisms with epithelial function, allowing experiments to distinguish effects associated with cellular orientation rather than treating the culture as an undifferentiated cell layer.
Tight junctions connect neighboring MDCK cells and help maintain the separation between apical and basolateral compartments. Their presence supports investigations of barrier function and selective transport, while changes observed during infection can be interpreted in relation to epithelial organization. This makes the monolayer useful for examining how host-pathogen interactions occur across an intact epithelial surface.
MDCK cells support analysis of several linked stages, including viral entry, replication, and release. Researchers can therefore use the same epithelial model to follow how infection begins, how efficiently the virus produces progeny within cells, and how newly produced virus exits the culture. Influenza studies are an important example of this broader infection-focused use.
A typical workflow begins by growing the cells until they form a confluent monolayer with organized epithelial surfaces. Researchers then expose the culture to a virus or antiviral compound and analyze infection-related outcomes, such as entry, replication, release, or compound sensitivity. The exact readout depends on whether the experiment emphasizes barrier behavior, viral biology, or screening.
These monolayers can reveal how infection relates to epithelial organization and whether viral processes are associated with the apical or basolateral side. They also provide a reproducible setting for examining viral propagation and for comparing responses to antiviral compounds. Such measurements help connect cellular infection events with the behavior of viruses in an epithelial barrier model.
Their reproducible growth and well-defined epithelial organization make MDCK cells suitable for screening studies and mechanistic comparisons. In an infection experiment, researchers can assess viral replication, release, or sensitivity to antiviral compounds under a consistent cellular arrangement. This supports both evaluation of candidate treatments and investigation of how epithelial structure influences host-pathogen interactions.