Primary Human Respiratory Epithelial Tissues
Primary Human Respiratory Epithelial Tissues - Related Videos
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
06:59
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
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Cited by 43 •
2013
The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.
11:46
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
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Cited by 29 •
2011
The fallopian tube (FT) is emerging as an alternative site of origin for serous ovarian carcinoma (SOC). This protocol describes a novel method for the isolation and ex vivo culture of fallopian tube epithelial cells. This system recapitulates the in vivo epithelium and allows the study of SOC pathogenesis.
06:33
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
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Cited by 132 •
2015
We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.
12:09
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
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Cited by 25 •
2013
The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.
FAQs
Tight junctions connect neighboring epithelial cells and help establish a continuous barrier, while mucus forms a surface layer that can interact with inhaled microbes. Together, these features allow researchers to examine whether infection disrupts barrier integrity, alters mucus production, or changes epithelial protection. This provides a more informative assessment of host defense than studying isolated cellular responses alone.
Mucociliary clearance links epithelial structure with physical removal of material from the airway surface. In differentiated tissues, mucus and epithelial activity can be studied alongside innate responses to pathogens, allowing investigators to evaluate both pathogen interaction and clearance-related defense. This is especially relevant when infection or inflammation changes the balance between protective airway function and epithelial injury.
These tissues preserve human airway features that may be simplified or altered in transformed cell lines, while providing a human-relevant complement to animal studies. Their physiological complexity supports examination of barrier function, mucus production, mucociliary clearance, and innate immunity in one system. Findings can therefore connect cellular mechanisms with responses that more closely reflect the human respiratory lining.
Culturing respiratory epithelial cells under an air–liquid interface promotes differentiation into airway epithelial cell types and enables formation of features associated with the respiratory lining. Researchers can then evaluate development of tight junctions, mucus production, mucociliary clearance, and innate responses. The resulting model supports experiments that depend on organized epithelial behavior rather than undifferentiated cell growth.
Pathogen exposure enables analysis of host–microbe interactions, including how epithelial barriers and innate responses change during infection. The same tissue system can support studies of viral and bacterial infection, helping investigators compare infection-related effects on mucus, barrier properties, and inflammatory activity. These observations contribute to disease modeling while retaining features of human respiratory tissue.
Primary human respiratory epithelial tissues allow researchers to connect infection with inflammatory changes and to examine how the epithelium responds to therapeutics. Because the model retains barrier, mucus, mucociliary, and innate-response features, investigators can assess treatment effects within a coordinated tissue context. This makes the system useful for studying respiratory disease mechanisms and evaluating responses in a human-relevant setting.