Artificial Anterior Chamber
Artificial Anterior Chamber - Related Videos
05:27
In Vivo Imaging of Liver Spheroids Engrafted in the Anterior Chamber of the Mouse Eye
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2024
Here, we describe a platform that allows noninvasive in vivo imaging of liver spheroids engrafted in the anterior chamber of the mouse eye. The workflow spans from generating spheroids from primary liver cells to transplantation into the mouse eye and in vivo imaging at cellular resolution by confocal microscopy.
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
08:21
Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy
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Cited by 2 •
2018
The goal of the protocol is to show longitudinal intravital real-time tracking of thymocytes by laser scanning microscopy in thymic implants in the anterior chamber of the mouse eye. The transparency of the cornea and vascularization of the graft allows for continuously recording progenitor cell recruitment and mature T-cell egress.
03:14
Artificial Selection - Concepts
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2019
Natural Selection and Adaptive Evolution In natural settings, adaptive evolution takes place by natural selection, a process in which differences in traits lead to differences in survival and reproductive success between individuals. This happens because individuals with certain phenotypes have a higher probability of survival and/or produce more surviving offspring than individuals with other phenotypes. A classic example of this was demonstrated by Rosemary and Peter Grant studying the...
05:54
Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time
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Cited by 31 •
2013
A new approach combining intraocular transplantation and confocal microscopy enables longitudinal, non-invasive real-time imaging with single-cell resolution within grafted tissues in vivo. We demonstrate how to transplant pancreatic islets into the anterior chamber of the mouse eye.
FAQs
Hydrostatic pressure allows researchers to adjust the forces acting on the isolated cornea while preserving a curved surface that remains optically relevant. This control helps examine how corneal tissue responds under different pressure conditions, making it possible to evaluate biomechanics and other tissue behaviors in a reproducible laboratory environment.
A curved corneal surface more closely represents the optical geometry of the eye than a flattened tissue sample. Preserving that shape supports evaluation of corneal behavior and procedures under conditions that remain relevant to ophthalmic research. The chamber therefore provides both mechanical control and an optically meaningful tissue configuration.
The setup supports investigation of corneal biomechanics, endothelial cell behavior, and wound healing. Because pressure and the surrounding fluid environment can be controlled, researchers can compare tissue responses under defined ex vivo conditions. These observations help characterize how the cornea behaves during laboratory studies without exposing a living patient to the experiment.
Preparation begins by positioning and sealing the corneal tissue over the chamber. The chamber is then filled with fluid, and hydrostatic pressure is adjusted to create the desired controlled environment. Maintaining the seal and pressure preserves the cornea's curved configuration, allowing subsequent testing of tissue behavior, surgical procedures, or ophthalmic devices.
Researchers can use the model to practice and evaluate procedures such as keratoplasty, a form of corneal surgery, without using a living patient. The isolated cornea provides a tissue surface on which surgical techniques can be examined under controlled pressure. This supports training and comparison of procedural approaches before clinical application.
The chamber can support assessment of surgical instruments, corneal implants, and techniques used in corneal procedures. Its reproducible ex vivo environment allows investigators to observe how these interventions interact with corneal tissue while controlling pressure and surface configuration. The resulting comparisons can inform experimental design and the refinement of ophthalmic approaches.