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Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy
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JoVE Journal Biology
Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

DOI:
10.3791/58236-v

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08:21 min

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October 02, 2018

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,
1School of Medicine and Dentistry,Universidad Católica de Valencia San Vicente Mártir, 2Unidad Mixta CIPF-UCV,Centro de Investigación Príncipe Felipe, 3Department of Medicine,University of Miami

Chapters

  • 00:04Title
  • 00:51Newborn Thymus Isolation
  • 02:13Thymus Anterior Eye Chamber Implantation
  • 04:073D Single Photon Fluorescence Implanted Thymus Confocal Imaging
  • 06:17Results: Representative In Vivo Thymus Implant Imaging
  • 07:11Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of the protocol is to show longitudinal intravital real-time tracking of thymocytes by laser scanning microscopy in thymic implants in the anterior chamber of the mouse eye. The transparency of the cornea and vascularization of the graft allows for continuously recording progenitor cell recruitment and mature T-cell egress.

Tags

In Vivo TrackingThymocytesAnterior ChamberLaser Scanning MicroscopyAutoimmunityImmunodeficiencyCentral ToleranceThymus InvolutionProgenitor RecruitmentT-cell EgressionThymus TransplantationCorneal IncisionAnterior Chamber Implantation

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