Biosensor Chips
Biosensor Chips - Related Videos
13:42
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
0 Views •
Cited by 9 •
2017
A microfluidic biosensor platform was designed and fabricated using low-cost dry film photoresist technology for the rapid and sensitive quantification of various analytes. This single-use system allows for the electrochemical readout of on-chip-immobilized enzyme-linked assays by means of the stop-flow technique.
14:43
Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
0 Views •
2013
We present a method for rapid, reversible immobilization of small molecules and functionalized nanoparticle assemblies for Surface Plasmon Resonance (SPR) studies, using sequential on-chip bioorthogonal cycloaddition chemistry and antibody-antigen capture.
04:43
β-Lactamase-Based Conductimetric Biosensor Assay for Protein-Protein Interactions
0 Views •
2025
This video demonstrates a conductimetric biosensor assay to study the interaction of an antibody with a target antigen immobilized on a transducer chip. The biosensor is engineered to have a bifunctional chimeric protein — a β-lactamase enzyme with a functional catalytic site fused to a nanobody for binding to the immobilized target antigen. Upon binding, the catalytic hydrolysis of an antibiotic substrate by the β-lactamase enzyme generates an electrical signal — confirming the...
15:27
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
0 Views •
Cited by 30 •
2017
We describe here protocols for the measurement of antibody-antigen binding affinity and kinetics using four commonly used biosensor platforms.
06:43
Graphene Biosensor-Based Field-Effect Biosensing to Detect Protein-Protein Interactions
0 Views •
2025
In this video, we describe the field-effect biosensing (FEB) technology to detect and quantify protein-protein interactions. The target proteins immobilized on the graphene biosensor interact with analyte proteins in solution, resulting in electrical current alterations in the FEB system. This change in the electrical response indicates the binding affinity between the proteins.
FAQs
The recognition element determines what a chip can measure because its antibody, enzyme, nucleic acid probe, or receptor interacts with a particular target. That binding event provides selectivity, while the transducer supplies the measurable output. Separating these roles allows the same basic chip architecture to support biomarker, pathogen, metabolite, or genetic-material analysis in medical testing.
Electrical, optical, and chemical outputs represent different ways of reporting recognition events or physiological information. After the transducer generates a response, integrated processing enables that signal to be interpreted as evidence of a target or biological state. This conversion connects molecular or physiological activity with a measurable result that can support medical analysis and decision-making.
Multiplexing allows a single chip to measure several targets or signals rather than limiting analysis to one measurement. In medicine, this can support combined assessment of biomarkers, pathogens, metabolites, or genetic material from a small sample. The approach broadens the information available from one analysis while preserving the compact format and reduced sample and reagent requirements described for these devices.
A typical analysis begins when a small sample or physiological signal reaches the chip. A recognition element interacts with the relevant molecule, or the device responds to the physiological input. The transducer then converts that event into an electrical, optical, or chemical signal, which integrated processing supports interpretation. The resulting information can be used for medical assessment.
Biosensor chips are useful when rapid information about biomarkers, pathogens, metabolites, or genetic material is needed from a small sample. Their compact format supports point-of-care testing, where measurements can be made closer to the patient and contribute to more timely clinical decisions. They can also support diagnosis, monitoring, and personalized care through targeted or multiplexed measurements.
These devices can provide measurements of biological targets that inform diagnosis or ongoing monitoring, including biomarkers, metabolites, pathogens, and genetic material. Because a chip may support multiplexed analysis, it can supply several related measurements from a small sample. That broader profile can contribute to personalized care by helping clinical decisions reflect the biological information available from an individual patient.