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Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
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JoVE Journal Chemistry
Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

DOI:
10.3791/50772-v

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14:43 min

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September 23, 2013

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, , , , , , ,
1Center for Systems Biology,Massachusetts General Hospital

Chapters

  • 00:05Title
  • 01:48Preparation of GST and Nanoparticle Conjugates
  • 03:48Surface Preparation
  • 05:07Monitoring On-chip Capture-cycloaddition of Functionalized Molecules
  • 06:26Monitoring Immobilization Density and Determination of Cycloaddition Rates
  • 08:27Measuring the Binding of FKBP12 to Immobilized AP1497
  • 10:49Results: Real-time Monitoring of On-chip Small Molecule Immobilization by Surface Plasmon Resonance
  • 13:51Conclusion

Summary

Automatic Translation

Automatic Translation

We present a method for rapid, reversible immobilization of small molecules and functionalized nanoparticle assemblies for Surface Plasmon Resonance (SPR) studies, using sequential on-chip bioorthogonal cycloaddition chemistry and antibody-antigen capture.

Tags

Keywords: MicrofluidicOn-chipCapture-cycloadditionReversible ImmobilizationSmall MoleculesMulti-component StructuresBiosensor ApplicationsTrans-cycloocteneTetrazineSurface Plasmon ResonanceNon-covalent ImmobilizationAntigen-antibodyAP1497FKBP12Nanoparticle

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