High-frequency Snare
High-frequency Snare - Related Videos
09:19
Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
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2012
We have developed a cell fusion assay that quantifies SNARE-mediated membrane fusion events by activated expression of β-galactosidase.
01:43
SNAREs and Membrane Fusion
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2023
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion. SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
10:58
SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
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Cited by 8 •
2016
Here, we present a protocol to detect single, SNARE-mediated fusion events between liposomes and supported bilayers in microfluidic channels using polarized TIRFM, with single molecule sensitivity and ~15 msec time resolution. Lipid and soluble cargo release can be detected simultaneously. Liposome size, lipid diffusivity, and fusion pore properties are measured.
00:51
What is a Frequency Distribution
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2023
A frequency is the number of times a value of the data occurs. The sum of all the frequency values represents the total number of students included in the sample. It is commonly used to group data of quantitative types. Frequency distributions can be displayed in a table, histogram, line graph, dot plot, or pie chart, just to name a few. A histogram is a graphical representation of tabulated frequencies, shown as adjacent rectangles, erected over discrete intervals (bins), with an area equal to...
01:11
Mean From a Frequency Distribution
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2023
Sometimes, data gathered from an experiment on a large sample or population are organized into concise tables. In such cases, the frequency of the quantitative data set is plotted in the form of a table. Or else, the data values are grouped into the quantity’s intervals, which form classes, and their respective frequencies are known. That is, the data values are distributed over different categories or classes. This is known as frequency distribution. When such a data set is encountered, the...
FAQs
The loop delivers high-frequency current to the targeted tissue, where electrical energy produces heat. That heat enables the wire to divide tissue while simultaneously coagulating small blood vessels. This combined cutting-and-coagulation action explains why the technique can remove a lesion and help limit bleeding during resection.
Lesion assessment determines whether the target can be approached and encircled appropriately, while controlled loop placement helps keep energy focused on intended tissue. These steps support effective resection because inaccurate targeting may compromise specimen acquisition or contribute to injury, including perforation, during gastrointestinal endoscopic procedures.
Histopathological evaluation provides information about the resected specimen after removal. That examination supports clinical interpretation of the lesion and helps distinguish the diagnostic value of the procedure from its therapeutic purpose. In this way, snare resection can both treat selected precancerous lesions and supply material for further assessment.
A practical sequence starts with lesion assessment, followed by controlled loop placement around the target and application of high-frequency current. The heat produced during activation cuts the tissue and coagulates small vessels. The resected tissue provides a specimen for histopathological evaluation, linking the technical procedure with both treatment and diagnostic review.
Clinicians use this approach most commonly for polyps and, in appropriate cases, for selected precancerous lesions. Its value extends beyond immediate removal: obtaining tissue for histopathology supports evaluation, while treating appropriate lesions can reduce the risk that they progress to colorectal cancer. The method therefore has diagnostic and preventive roles.
Both complications require attention during resection. Coagulation of small blood vessels can help control bleeding, but careful lesion assessment, controlled loop placement, and focused tissue removal remain important. Perforation is another potential complication, so clinicians must maintain precise technique and manage adverse events carefully throughout the procedure.