Tear Fluid
Tear Fluid - Related Videos
08:16
Phenol Red Thread-based Sampling Procedure for Untargeted Tear Fluid Lipidomics in Biomarker Discovery
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2025
This protocol describes a unique clinical protocol for collecting human tear fluid samples using phenol red threads and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based workflow to discover the tear lipidomic profile. The simple methyl tert-butyl ether (MTBE)/methanol biphasic separation method enables rapid tear lipid extraction with high recovery for tear biomarker discovery.
07:45
Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy
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Cited by 1 •
2025
This article describes a protocol for the collection and detection of miR-15a from tears as a new diagnostic tool for diabetic retinopathy.
05:57
Sex Hormone Detection In Human Tear Film Using Enzyme-Linked Immunosorbent Assays
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2026
This protocol describes an optimized enzyme-linked immunosorbent assay (ELISA) method to detect and quantify 17β-estradiol and testosterone in human tear fluid collected via microcapillary tubes. This approach enables minimally invasive measurement of tear hormone levels.
04:38
A Novel Technique of Rescuing Capsulorhexis Radial Tear-out using a Cystotome
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Cited by 4 •
2011
Capsulorhexis is an important step in phacoemulsification surgery. A surgeon creates a continous curvilinear tear on the anterior lens capsule by controlling the tearing vector forces. A peripherally extended tear is a serious complication. This video demonstrates a novel technique of rescuing capsular radial tear out using a cystotome.
09:22
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
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Cited by 12 •
2017
Analysis of tear film cytokines helps in studying various ocular diseases. Bead based multiplex assays are simple and sensitive and enable the testing of multiple targets in samples with small volumes. Here we describe a protocol for tear film cytokine profiling a using bead based multiplex assay.
FAQs
Its layered organization assigns different jobs across the ocular surface. The aqueous component supports hydration, while mucins help organize and stabilize the surface, and lipids contribute to the film that supports optical quality. Considering these components together helps explain why altered composition can affect comfort or visual performance.
Blinking distributes tear fluid across the eye, helping maintain an even protective film and spread its lubricating components over the cornea. Excess fluid then leaves through the lacrimal drainage system. This coordinated movement supports surface cleanliness by helping remove particles while preventing fluid from accumulating on the eye.
Both the amount of tear fluid and its molecular makeup can provide information about ocular-surface health. Changes may accompany dry eye and other disorders, so examining these features can help connect symptoms or surface abnormalities with potential disease-related changes. The findings may also support assessment of treatment effects over time.
Tear-fluid analysis can contribute to diagnosis, treatment assessment, and investigation of disease-related biomarkers. Researchers and clinicians may examine whether measurable changes in tear volume or composition correspond with ocular-surface disorders or treatment responses. Because the sample reflects material present at the eye, it also supports research into noninvasive approaches to disease assessment.
Treatment assessment can include examining whether tear volume or composition changes after an intervention. These measurements provide an ocular-surface perspective that complements clinical evaluation, particularly when studying dry eye or related disorders. Tracking such changes may help determine whether therapy is associated with improvement, persistence, or alteration of tear-film abnormalities.
Tear fluid is being investigated as a source of noninvasive biomarkers for both ocular and systemic disease. Its relevance extends beyond lubrication because changes in its measurable properties may provide disease-related information without relying solely on more invasive sampling. This makes tear analysis useful in medicine and ocular-surface research.