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Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
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Immunology and Infection
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JoVE Journal Immunology and Infection
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

DOI:
10.3791/55993-v

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09:22 min

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October 13, 2017

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, , , , , ,
1National Healthcare Group Eye Institute,Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), 3Institute of Molecular and Cell Biology,A*STAR, 4GROW Research Laboratory,Narayana Nethralaya Foundation, 5Vimta Labs Limited

Chapters

  • 00:05Title
  • 00:42Elution of Tear Sample from Tear Flow Strip
  • 01:26Cytokine Profiling by Bead Based Multiplex Assay
  • 04:43Reading the Assay Plate and Data Analysis
  • 06:56Results: Tear Cytokine Profiles from Four Male Subjects
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

Analysis of tear film cytokines helps in studying various ocular diseases. Bead based multiplex assays are simple and sensitive and enable the testing of multiple targets in samples with small volumes. Here we describe a protocol for tear film cytokine profiling a using bead based multiplex assay.

Tags

Keywords: Tear Cytokine ProfileBead-based Multiplex AssaySchirmer StripOphthalmologyCytokinesOcular DiseasesAnalytesTear SampleAssay BufferCentrifugationCytokine ProfilingMultiplex AssayCytokine StandardsAssay WorksheetMagnetic Plate Washer

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