Electrophysiology Calcium Imaging
Electrophysiology Calcium Imaging - Related Videos
10:30
Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging
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Cited by 17 •
2017
Once removed from the body, neuronal tissue is greatly affected by environmental conditions, leading to eventual degradation of the tissue after 6 - 8 h. Using a unique incubation method, which closely monitors and regulates the extracellular environment of the tissue, tissue viability can be significantly extended for >24 h.
09:42
Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
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Cited by 9 •
2013
Procedures are described to perform simultaneous recordings of membrane potential or current and changes of intracellular calcium concentration. Suprachiasmatic nucleus neurons are filled with the calcium indicator bis-fura-2 using a patch clamp electrode in the whole cell patch clamp configuration.
08:43
Calcium Imaging in Neurons
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2023
Calcium ions play an integral role in neuron function: They act as intracellular signals that can elicit responses such as altered gene expression and neurotransmitter release from synaptic vesicles. Within the cell, calcium concentration is highly dynamic due to the presence of pumps that selectively transport these ions in response to a variety of signals. Calcium imaging takes advantage of intracellular calcium flux to directly visualize calcium signaling in living neurons.This video begins...
11:06
In vivo Neuronal Calcium Imaging in C. elegans
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Cited by 20 •
2013
With its small transparent body, well-documented neuroanatomy and a host of amenable genetic techniques and reagents, C. elegans makes an ideal model organism for in vivo neuronal imaging using relatively simple, low-cost techniques. Here we describe single neuron imaging within intact adult animals using genetically encoded fluorescent calcium indicators.
16:33
Functional Calcium Imaging in Developing Cortical Networks
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Cited by 32 •
2011
Spontaneous activity of developing neuronal networks can be measured using AM-ester forms of calcium-sensitive indicator dyes. Changes in intracellular calcium, indicating neuronal activation, are detected as transient changes in indicator fluorescence with one- or two-photon imaging. This protocol can be adapted for a range of developmentally-dependent neuronal networks in vitro.
FAQs
The pairing lets researchers align rapid electrical events with slower intracellular calcium changes in the same cells. This reveals whether membrane voltage or current activity is accompanied by a cellular signaling response, rather than requiring separate experiments to infer that relationship at the cellular level. The comparison therefore connects neuronal activity with its intracellular consequence.
Fluorescent calcium indicators change their signal when they bind Ca2+. In an active neuron, that signal can therefore report calcium entering the cell or being released inside it. Because the optical response tracks calcium rather than membrane voltage directly, comparing both readouts helps distinguish electrical activity from its intracellular signaling consequence.
Electrical recordings capture membrane voltage or current directly, whereas calcium fluorescence changes on a slower timescale. Comparing the traces can show how synaptic inputs and action potentials relate to intracellular calcium dynamics. This temporal contrast is important when interpreting whether a calcium signal reflects an immediate electrical event or a downstream cellular response.
A typical experiment uses patch-clamp recording together with fluorescence microscopy. Researchers record membrane voltage or current from an individual neuron while monitoring indicator fluorescence in that cell. They then compare the electrical trace with the calcium signal over time, preserving a direct link between cellular activity and the optical response.
The combined readout can characterize responses in individual neurons or across populations while retaining spatial information from fluorescence imaging. It can connect synaptic inputs and action potentials with cellular calcium responses, helping investigators examine how activity is distributed among cells rather than relying only on a bulk measure.
In neuroscience, this approach supports studies of circuit function, neuronal development, and disease mechanisms. Its value comes from linking electrical activity to spatially resolved calcium responses, so investigators can examine cellular signaling within broader circuits. The method is especially relevant when a research question requires both functional activity and its intracellular consequence.