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Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging
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Chapters
- 00:05Title
- 01:11Brain Slice and Retinal Wholemount Preparation
- 04:02Calcium Dye Loading
- 05:38Electrophysiological Recordings and Imaging
- 07:28Results: Electrophysiological and Calcium Recordings in Retinal Wholemount
- 08:49Conclusion
Once removed from the body, neuronal tissue is greatly affected by environmental conditions, leading to eventual degradation of the tissue after 6 - 8 h. Using a unique incubation method, which closely monitors and regulates the extracellular environment of the tissue, tissue viability can be significantly extended for >24 h.
