Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging

Morven A. Cameron; Orsolya Kekesi; John W. Morley; Alba Bellot-Saez; Sindy Kueh; Paul Breen; Andr&#233; van Schaik; Jonathan Tapson; Yossi Buskila

doi:10.3791/55396

Journal

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Neuroscience

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Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging

12,014 Views

•

10:30 min

•

February 15, 2017

DOI:

10.3791/55396-v

DOI:

10.3791/55396-v

•

10:30 min

February 15, 2017

•

11954 Views

Morven A. Cameron¹, Orsolya Kekesi^1,2, John W. Morley^1,2, Alba Bellot-Saez^1,2, Sindy Kueh², Paul Breen¹, André van Schaik¹, Jonathan Tapson¹, Yossi Buskila^1,2

¹The MARCS Institute,Western Sydney University ²School of Medicine,Western Sydney University

Summary

Once removed from the body, neuronal tissue is greatly affected by environmental conditions, leading to eventual degradation of the tissue after 6 - 8 h. Using a unique incubation method, which closely monitors and regulates the extracellular environment of the tissue, tissue viability can be significantly extended for >24 h.