Mouse Brain Injection
Mouse Brain Injection - Related Videos
04:58
Brain Metastasis Mouse Model: A Method to Generate Brain Metastasis Mouse Model by Intracarotid Cancer Cell Injection
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2025
This video demonstrates the establishment of a brain metastasis mouse model by intracarotid injection of fluorescently labeled cancer cells. The mouse model can be used to study the effects of novel therapeutics and the underlying physiological mechanisms.
07:43
Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis
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Cited by 32 •
2017
Brain metastasis has become an urgent unmet medical need as its incidence has increased while therapeutic options have remained palliative. Creating experimental animal models of brain metastasis via intracarotid arterial injection of cancer cells facilitates mechanistic studies of the disease biology and evaluation of novel intervention regimens.
05:01
Injectable Mesh Electronics for Stable Single-Neuron Recordings in a Mouse Brain
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2025
The video demonstrates the injection of mesh electronics probes into a mouse brain for stable long-term recordings at a single-neuron level. An anesthetized mouse with an exposed skull is secured on a stereotaxic frame, and the probe's mesh device region is injected into the brain through drilled holes. The probe's input-output pads are connected to a circuit, and recordings are obtained using a data acquisition system.
02:59
An Intracerebroventricular Injection to Analyze Murine Cytomegalovirus Distribution in a Neonatal Mouse Brain
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2025
This video demonstrates the intracerebroventricular injection process for studying murine cytomegalovirus (MCMV) distribution in the neonatal mouse brain. MCMV, engineered to express an enhanced green fluorescent protein (EGFP) for visibility, is injected into the lateral ventricle of a mouse pup's brain. The injected virus circulates through the cerebrospinal fluid, infecting cells in the periventricular region, including the marginal zone, choroid plexus, and subventricular zone.
08:28
Zebrafish Brain Ventricle Injection
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Cited by 72 •
2009
After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.
FAQs
Head stabilization provides a fixed reference while the injection needle is guided to selected brain coordinates. This combination links the intended anatomical target with the actual deposition site, helping researchers study defined neural regions rather than relying on less controlled placement. Consistent targeting is therefore central to comparing neural structure or function across experimental animals.
The injected material helps determine how the experiment affects nearby tissue. Drugs, tracers, viral vectors, and cell suspensions serve different experimental purposes, while diffusion and local biological responses vary with both the substance and the injection site. These differences influence whether the primary result concerns neural activity, anatomical tracing, gene manipulation, or cellular effects.
Injection site and measured volume are important variables because they define where and how much material enters the brain. The resulting distribution is not uniform across all experiments: diffusion and local biological responses depend on the material and selected region. Controlling these variables helps relate observed changes to the intended neural target.
Careful targeting limits variation in the region receiving the substance, while appropriate tissue handling helps preserve the experimental material and the local biological context for later study. Together, these practices improve reproducibility, making results easier to compare across animals and strengthening conclusions about relationships between an injected substance and neural structure or function.
A typical workflow stabilizes the animal’s head, uses stereotaxic coordinates to guide a needle toward the selected brain region, and deposits a measured volume through the skull. The injected substance and target site are then considered when interpreting diffusion and local responses. Consistent tissue handling supports reliable downstream evaluation of the experiment.
Researchers can select the injected substance according to the question being studied. Tracers support examination of neural organization, whereas viral vectors can enable gene manipulation; drugs or cell suspensions provide other controlled interventions. Delivering these materials to a defined region allows investigators to connect local treatment with neural circuits, structure, or function.
The technique provides a controlled way to place a drug, viral vector, or cell suspension in a selected brain region. This makes it useful for modeling disease-related changes and evaluating therapeutic strategies under defined anatomical conditions. Outcomes can be examined in relation to the target site, material delivered, diffusion pattern, and local biological response.