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Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis
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JoVE Journal Cancer Research
Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis

Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis

DOI:
10.3791/55085-v

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07:43 min

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February 08, 2017

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, ,
1Department of Molecular and Cellular Oncology,The University of Texas MD Anderson Cancer Center

Chapters

  • 00:05Title
  • 00:56Prepare Cancer Cells for Injection
  • 02:15Prepare Mice for Tumor Cell Injection
  • 04:02Cancer Cell Injection into Carotid Artery
  • 05:44Results: Non-invasive Monitoring for Brain Metastasis Outgrowth
  • 06:25Conclusion

Summary

Automatic Translation

Automatic Translation

Brain metastasis has become an urgent unmet medical need as its incidence has increased while therapeutic options have remained palliative. Creating experimental animal models of brain metastasis via intracarotid arterial injection of cancer cells facilitates mechanistic studies of the disease biology and evaluation of novel intervention regimens.

Tags

Keywords: Brain MetastasisCancer Cell InjectionCarotid ArteryIn Vivo Experimental ModelMouse ModelMicrosurgical TechniqueReproducibilityTherapeutic EfficacyCell CultureCell HarvestingCell ResuspensionAnesthesiaSurgical ProcedureCarotid Artery IsolationBlood Flow Occlusion

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