Oligodendrocyte Isolation
Oligodendrocyte Isolation - Related Videos
03:06
Isolating Murine Primary Oligodendrocytes Using Immunomagnetic Beads
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2025
This video demonstrates a method to isolate primary oligodendrocyte cells from a neural cell suspension obtained from a mouse pup using magnetic separation. The technique employs magnetic beads coated with antibodies specific to oligodendrocyte antigens, enabling selective isolation of these cells.
03:59
Isolation and Purification of Recombinant Myelin Oligodendrocyte Glycoproteins
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2025
Source: Jain, R. W., et al. Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies. J. Vis. Exp. (2016)This video demonstrates the isolation and purification of MOGtag, a recombinant Myelin Oligodendrocyte Glycoprotein (MOG) used in Experimental Autoimmune Encephalomyelitis (EAE) studies. The protocol involves bacterial lysis, sonication, centrifugation, and nickel affinity chromatography to obtain...
04:39
Isolating and Culturing Oligodendrocyte Precursor Cells from Mouse Pup Brain Cortex
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2025
This video demonstrates a technique for isolating and culturing oligodendrocyte precursor cells (OPCs) from the mouse pup brain cortex.
18:17
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues
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Cited by 103 •
2011
This article describes methods to derive enriched populations of murine oligodendrocyte precursor cells (OPCs) in primary culture, which differentiate to produce mature oligodendrocytes (OLs). In addition, this report describes techniques to produce murine myelinating co-cultures by seeding mouse OPCs onto a neurite bed of mouse dorsal root ganglion neurons (DRGNs).
02:01
A Method for Generating Oligodendrocyte-Conditioned Medium
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2025
This video demonstrates the production of oligodendrocyte-conditioned medium (OCM) from oligodendrocyte lineage cells by stressing them to enhance their molecular release into the medium. This medium, which is rich in growth factors, cytokines, and other bioactive molecules, can be added to neuron cultures to gain insight into the impact of oligodendrocytes-secreted factors on neuronal physiology and connectivity.
FAQs
They provide two distinct strategies for enriching oligodendrocytes after tissue dissociation. Density-gradient centrifugation separates cellular material through a gradient, whereas antibody-based selection uses antibody recognition to select target cells. The choice affects how the preparation is enriched for downstream studies of myelination, progenitor differentiation, neuron-glia interactions, or responses to inflammatory signals.
The two dissociation approaches work together to convert brain or spinal cord tissue into a cell preparation suitable for separation. Enzymatic and mechanical processing provide complementary ways to disrupt the tissue before enrichment. Their inclusion is important because the resulting cells must remain suitable for culture-based investigations of survival, maturation, myelin production, and neural support.
Post-isolation culture conditions determine whether the recovered cells remain viable and can mature sufficiently for analysis. Conditions that support survival and maturation allow researchers to examine progenitor differentiation and myelin production rather than only the initial separated population. This makes culture an important stage for connecting cell preparation with functional neuroscience questions.
A typical workflow begins with brain or spinal cord tissue, followed by enzymatic and mechanical dissociation. The resulting preparation then undergoes cell separation, using density-gradient centrifugation or antibody-based selection. Finally, the separated cells are placed in culture conditions that support survival and maturation, enabling subsequent studies of myelination, disease-related responses, or neuron-glia interactions.
Researchers use these preparations when they need to examine oligodendrocyte behavior outside intact nervous tissue. Applications include studying myelination, neural support, progenitor differentiation, and interactions between neurons and glia. The approach also supports disease-focused work on multiple sclerosis and leukodystrophies, as well as investigations of remyelination and possible cell-based repair strategies.
Isolated cells can be evaluated for myelin production, maturation, and responses to injury-associated or inflammatory signals. These observations help connect cellular behavior with processes relevant to remyelination and nervous-system disease. Because the preparation can be cultured, researchers can examine oligodendrocyte responses and neuron-glia relationships under defined experimental conditions rather than relying only on tissue-level observations.