Oxidative Current Measurement
Oxidative Current Measurement - Related Videos
07:28
Measurement of Bioelectric Current with a Vibrating Probe
0 Views •
Cited by 15 •
2011
The manufacture, calibration and use of non-invasive vibrating probes to measure bioelectric current in various biological systems is described.
09:55
Preparation of Janus Particles and Alternating Current Electrokinetic Measurements with a Rapidly Fabricated Indium Tin Oxide Electrode Array
0 Views •
Cited by 2 •
2017
In this article, a simple method to prepare partially or fully coated metallic particles and to perform AC electrokinetic property measurements with a rapidly fabricated indium tin oxide (ITO) electrode array is demonstrated.
08:23
Comet Assay as an Indirect Measure of Systemic Oxidative Stress
0 Views •
Cited by 23 •
2015
Comet assay measures DNA breaks, induced by different factors. If all factors (except oxidative stress) causing DNA damage are kept constant, the amount of DNA damage is a good indirect parameter of oxidative stress. The goal of this protocol is to use comet assay for indirect measurement of oxidative stress.
02:31
Measuring the Kinetic Isotope Effect on Microbial Electron Transport Using Deuterium Oxide
0 Views •
2025
Source: Tokunou, Y., et al, Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1. J. Vis. Exp. (2018)This video demonstrates a whole-cell electrochemical method to assess how proton transfer affects extracellular electron transport (EET) in electroactive bacteria. By replacing protons with deuterons using deuterium oxide, the method measures changes in current to reveal a kinetic isotope effect, highlighting the role of...
04:25
Substrate Oxidation Assay in Cultured Cells: An In Vitro Assay to Quantify Substrate Oxidation in Cells by Measuring Radioactive Signals from Trapped CO2
0 Views •
2025
This video demonstrates a quantification technique for CO2 released during substrate oxidation using 14C-radiolabeled substrates. The released 14CO2 is trapped in an alkaline solution and quantified by a scintillation counter. The oxidation of different substrates varies between tissues and reflects the pathophysiological condition of the tissue.
FAQs
An imposed, controlled potential provides the driving force for electron transfer from an electroactive molecule to the electrode. When dopamine reaches the electrode surface, its oxidation contributes to the measured current. Because the potential is controlled, researchers can relate changes in current over time to changes in the amount of molecule arriving at that surface.
Electrode design and calibration strongly influence measurement specificity and accuracy. Design affects how selectively the electrode responds to electroactive molecules, while calibration establishes how measured current corresponds to the amount reaching the electrode. These factors are essential when interpreting chemical signals in complex environments such as brain tissue, where reliable assignment of current changes matters.
Neurotransmitter release can change rapidly as neuronal activity occurs, so temporal sensitivity helps resolve chemical events over time rather than only indicating an accumulated signal. Oxidative current measurement can therefore connect fluctuations in dopamine or other electroactive molecules with neuronal activity, synaptic transmission, and circuit-level signaling in brain tissue.
A typical workflow places an electrode in the relevant sample or brain tissue, applies a controlled potential, and records the resulting current as molecules undergo oxidation at the electrode surface. Researchers then use calibration and electrode characteristics to interpret the signal. Tracking current over time provides information about changing molecular arrival at the electrode.
In neuroscience, the approach can monitor rapid neurotransmitter release in brain tissue and examine how chemical signaling relates to neuronal activity. Its measurements support studies of synaptic transmission, neuromodulation, and circuit function. Researchers can also apply it to investigate disease-related changes in chemical signaling when electrode performance supports accurate detection.
A current change reflects the amount of an electroactive molecule reaching the electrode surface over time, not simply a general measure of neuronal activity. Interpretation therefore depends on electrode specificity, calibration, and the experimental context. With those factors considered, the signal can help relate chemical release to neuronal activity and circuit function.