Spiral Acquisition
Spiral Acquisition - Related Videos
02:35
Culturing Murine Spiral Ganglion Neuron Explants on Multielectrode Arrays
0 Views •
2025
This video demonstrates the process for isolating spiral ganglion explants from the inner ear and co-culturing them with the organ of Corti on a multielectrode array. Spiral ganglia are carefully extracted and sectioned into smaller tissue sections or explants. These explants are cultured on the multielectrode array's active area to promote neuronal process outgrowth.
03:51
Label-Free Neutrophil Separation from Tracheal Secretion Using Spiral Microfluidics Channel
0 Views •
2025
This video demonstrates separating neutrophils from tracheal secretions using a microfluidic device with spiral channels. The device achieves efficient separation based on cell size by utilizing varying thicknesses and trapezoidal-shaped channels. The interplay between forces results in large neutrophils accumulating near the inner wall while erythrocytes and mucin aggregates remain near the outer wall, ensuring effective neutrophil separation.
07:51
Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays
0 Views •
Cited by 5 •
2016
We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.
02:40
Analyzing Neural Activity in Primary Murine Spiral Ganglion Neurons Using Multielectrode Arrays
0 Views •
2025
This video demonstrates the recording of spontaneous and stimulation-dependent neural activity from primary murine spiral ganglion neuron (SGN) cultures on a multielectrode array (MEA). It involves washing and preparing the MEA, recording spontaneous neural activity, and applying controlled electrical stimuli to identify stimulation-dependent neuronal activity.
05:14
Differentiating Immortalized Multipotent Otic Progenitors into Spiral Ganglion Neurons and Evaluating the Differentiation
0 Views •
2025
The video demonstrates the differentiation of immortalized multipotent otic progenitors (iMOPs) into spiral ganglion neurons (SGNs) and immunofluorescence-based confirmation of the differentiation. The iMOPs are plated onto culture substrate-coated coverslips in a neuronal differentiation medium for differentiation into SGNs. The differentiated cells are labeled with antibodies specific for neuronal differentiation markers and analyzed under a microscope to confirm differentiation.
FAQs
Rapidly varying magnetic-field gradients control how the MRI signal traverses k-space during a readout. Their waveform moves the sampling path continuously from central spatial-frequency information toward outer regions, allowing two-dimensional coverage within one readout. This coordinated gradient control produces the technique’s acquisition efficiency and supports measurements of rapidly changing neural signals.
Spiral acquisition can shorten the time required to collect the spatial-frequency data needed for an image because it samples two dimensions through one continuous gradient waveform rather than separate Cartesian lines. Shorter readouts allow images or measurements to be repeated more rapidly, which is particularly useful when the signal changes during functional or other dynamic neuroscience experiments.
Magnetic-field inhomogeneity and off-resonance can cause the recorded signal to accumulate phase inconsistently as it follows the spiral trajectory. The resulting image may appear blurred or geometrically distorted rather than simply reduced in intensity. Because these effects arise during the time-varying readout, accurate image formation requires specialized reconstruction and correction procedures.
After the readout, the sampled spatial-frequency information must be used to form an image through a reconstruction process. For Spiral acquisition, reconstruction is especially important because off-resonance, magnetic-field inhomogeneity, and motion can alter how the data correspond to image locations. Applying appropriate correction during reconstruction helps reduce blurring and distortion in the resulting images.
Researchers may choose Spiral acquisition when the experiment requires rapid sampling of changing brain signals. Its shortened readouts can support fast functional MRI and other dynamic measurements, helping track temporal changes in brain activity. The approach is therefore most relevant when acquisition efficiency and temporal resolution are important outcomes, provided that reconstruction can address its sensitivity to artifacts.
Interpretation should account for the balance between faster data collection and greater sensitivity to image-degrading effects. Magnetic-field inhomogeneity, off-resonance, and subject motion may produce blurring or distortion, potentially affecting apparent patterns of brain activity. Investigators should therefore consider the effectiveness of specialized reconstruction and correction when evaluating dynamic neuroscience measurements obtained with this technique.