Oxygenated Saline
Oxygenated Saline - Related Videos
01:17
Salinity Tolerance of Alishewanella Species Isolated from Seawall Muddy Water
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2025
Begin with a culture of Alishewanella, a Gram-negative, salt-tolerant bacterium.Next, streak the culture onto agar plates containing increasing concentrations of sodium chloride to simulate varying levels of saline stress.Overlay each plate with a layer of unsolidified medium to prevent oxygen diffusion and maintain the culture in an oxygen-limited environment.Seal and incubate the plates at an optimum temperature to support bacterial growth.During incubation, the bacterium exhibits varying...
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cited by 28 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
03:15
A Hypertonic Saline Injection Technique for Inducing Glaucoma in Rats
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2025
This video demonstrates glaucoma induction in an anesthetized rat by injecting hypertonic saline into the episcleral vein. The saline enters the trabecular meshwork via Schlemm's canal, causing tissue scarring, which blocks aqueous humor outflow and raises intraocular pressure. The elevated pressure damages retinal ganglion cells, leading to glaucoma.
09:08
Detecting Reactive Oxygen Species
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2023
Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...
06:29
Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs
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Cited by 2 •
2016
Embryonic stages are the most susceptible to xenobiotics. Although chemical toxicity depends on salinity, no method exists to test the salinity dependence of toxicity to aquatic organisms. Here, we describe a new and high-throughput method for determining the salinity dependence of toxicity to aquatic embryos.
FAQs
In excised neural tissue, dissolved oxygen can diffuse from the surrounding solution toward cells. This compensates for the loss of blood circulation, which otherwise limits oxygen delivery after tissue removal. Maintaining that supply supports cellular metabolism, helping preparations remain suitable for functional measurements outside the intact organism.
Its ionic composition, pH, and osmolarity establish the extracellular environment surrounding the tissue. Oxygenation alone would not maintain the chemical conditions required for physiological neuronal function. Controlling these properties helps researchers attribute observed changes to the neural process under study rather than to unintended changes in the bathing solution.
Bubbling an oxygen-containing gas through saline increases the opportunity for oxygen to enter the aqueous phase. The dissolved oxygen then becomes available for diffusion through the preparation. This approach adds oxygen while preserving the solution's intended ionic, pH, and osmotic conditions as part of the experimental control.
Its value is especially apparent when circulation is absent but the tissue must remain metabolically active. In acute brain slices and other excised preparations, oxygen availability helps sustain neuronal function long enough to examine electrical activity and synaptic behavior. It supports investigations of neural mechanisms in a controlled, reduced system.
A basic preparation workflow begins with an aqueous saline whose extracellular properties are controlled, followed by bubbling with an oxygen-containing gas. Dissolved oxygen is allowed to distribute through the solution before the oxygenated solution contacts tissue. This creates the bathing or perfusion environment used to support isolated preparations during experimentation.
This solution can be used with acute brain slices, tissue preparations, and perfusion-based experiments. It enables electrophysiological recordings, synaptic studies, and investigations of neural circuits while the tissue remains outside the organism. These applications allow measured activity to be examined under controlled extracellular conditions rather than under intact circulation.