Stab Wound Model
Stab Wound Model - Related Videos
09:16
Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration
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Cited by 60 •
2014
To shed light on the cellular and molecular mechanisms of zebrafish adult neurogenesis and regeneration, we developed a protocol for invasive surgery causing mechanical injuries in the zebrafish adult telencephalon and subsequent monitoring of changes in the stabbed hemisphere by immunohistochemistry or in situ hybridization.
04:03
Stab-Wound Mouse Model for Studying Hemorrhage and Inflammation in Traumatic Brain Injury
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Cited by 1 •
2025
Simplified traumatic brain injury (TBI) models have facilitated the development of therapeutic approaches. This protocol outlines the creation of a stab-wound mouse cortex using needles, enabling the analysis of hemorrhage and inflammation. The stab-wound TBI mouse model offers the advantage of being performed without requiring specialized equipment.
08:35
Demonstration of the Rat Ischemic Skin Wound Model
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Cited by 32 •
2015
The rat, due to its size, availability, and rather docile behavior, has been utilized as a research model for many years. The goal of this protocol is to utilize the rat as an ischemic skin wound healing model to provide valuable insight into the pathophysiology of chronic wounds.
05:39
Murine Model of Wound Healing
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Cited by 207 •
2013
A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.
06:12
Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity
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Cited by 3 •
2022
A mechanical brain injury model in the adult zebrafish is described to investigate the molecular mechanisms regulating their high regenerative capacity. The method explains to create a stab wound injury in the optic tectum of multiple species of small fish to evaluate the regenerative responses using fluorescent immunostaining.
FAQs
Penetration physically disrupts neural tissue and nearby cells, while damage to local blood vessels compromises the blood-brain barrier. These events create a localized lesion that can trigger neuroinflammation, glial activation, and tissue remodeling. Because the injury is spatially defined, investigators can examine how damage develops within the lesion and how nearby neural tissue responds.
Blood-brain barrier compromise is an important consequence of vessel damage because it marks a change in the interface between neural tissue and the surrounding circulation. In the Stab Wound Model, this disruption can be examined alongside inflammatory signaling and glial responses. Measuring these linked changes helps researchers characterize how focal trauma alters the local brain environment.
The model supports analysis of several connected responses, including neuronal loss, neuroinflammation, glial activation, and tissue remodeling around the lesion. Investigators can also examine injury progression and inflammatory signaling in the affected region. Together, these outcomes provide a framework for studying both the damaging effects of trauma and the mechanisms associated with local repair.
A controlled penetration injury produces a lesion in a specified neural area, allowing investigators to relate observed cellular and tissue changes to a localized site of trauma. This defined arrangement supports comparisons among the lesion, surrounding tissue, and broader neural network. Its reproducible framework is particularly valuable when researchers need to follow focal injury progression or assess regional responses.
Researchers can evaluate neuronal loss, inflammatory signaling, glial activation, and tissue remodeling in and around the lesion. The approach also allows assessment of injury progression and changes associated with repair mechanisms. Examining these outcomes together can reveal how localized mechanical damage affects both the injured tissue and the surrounding neural network.
It is useful when a study needs a reproducible focal traumatic brain injury framework for testing whether an intervention limits damage or supports repair. Researchers can compare treatment-associated changes in neuronal loss, inflammatory responses, glial activation, or tissue remodeling. The model therefore connects therapeutic evaluation with defined biological outcomes in a localized brain region.